Subtypes of HLA-A1 defined on the basis of CTL precursor frequencies |
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Authors: | L Zhang T F Rinke de Wit S G Li J J van Rood F H Claas |
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Institution: | Department of Immunohaematology, Leiden University Hospital, The Netherlands. |
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Abstract: | Recently we have shown that limiting dilution analysis can be used to detect cytotoxic T-cell precursor frequencies directed against individual HLA class I antigens. Using the same protocol, we have been able to define two subtypes of HLA-A1, which are indistinguishable by conventional typing sera as well as by cell-mediated lympholysis. One-dimensional isoelectric focusing analysis of the variants did not show any overall charge differences. However, family studies indicated that these HLA-A1 subtypes are genetically determined and can be distinguished on the bases of T-cell precursor frequencies in HLA-A1-negative blood donors. |
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