细辛水提物与尼莫地平合用的镇痛作用及其机制研究

目的 研究细辛水提物和钙通道拮抗剂尼莫地平合用的镇痛作用及其机制。方法 40只健康Wistar雄性大鼠随机分为对照组、模型组、细辛组、尼莫地平组和联合用药组，除对照组外其他各组大鼠均进行结扎坐骨神经制备神经病理性疼痛模型。各组大鼠手术后ig给药2周，细辛组大鼠给予细辛水提物（200 mg/kg，相当于细辛药材2 g/kg），尼莫地平组大鼠给予尼莫地平（40 mg/kg），联合给药组大鼠给予尼莫地平（40 mg/kg）30 min后给予细辛水提物（200 mg/kg），对照组和模型组给予生理盐水。各组大鼠分别于手术前1 d及手术后第1、3、5、9、14 天给药30 min后分别测量热缩足潜伏期以及机械刺激缩足反射阈值。结果 各组大鼠手术前的热缩足潜伏期以及机械刺激缩足反射阈值的差异无统计学意义(P＞0.05)，手术后各时间点模型组大鼠的热缩足潜伏期和机械刺激缩足反射阈值均较对照组显著降低（P＜0.05、0.01），细辛组和尼莫地平组大鼠的热缩足潜伏期和机械刺激缩足反射阈值均从术后第5天起较模型组大鼠显著升高（P＜0.05、0.01），联合用药组大鼠热缩足潜伏期和机械刺激缩足反射阈值从手术后第3天起均较模型组大鼠显著升高（P＜0.05、0.01），从手术后第5天起均较细辛组和尼莫地平组高（P＜0.05、0.01）。联合用药组的镇痛作用强于细辛水提物和尼莫地平。结论 细辛水提物和尼莫地平合用具有镇痛作用。

Study on analgesic effects of aqueous extract from Asari Radix et Rhizoma combined with Nimodipine and its mechanism

Objective To study the analgesic effects of aqueous extract from Asari Radix et Rhizoma (ARR) combined with Nimodipine and its mechanism. Methods Forty healthy male Wistar rats were randomly divided into control, model, ARR, Nimodipine, and ARR + Nimodipine groups. Except the control group, the neuropathic pain models of rats were produced in the rest groups by the ligation of sciatic nerve. Rats in each group were ig administered for two weeks after operation. The aqueous extract (200 mg/kg) from ARR was given to rats in ARR group, Nimodipine (40 mg/kg) was given to rats in Nimodipine group, and the aqueous extract (200 mg/kg) from ARR was given to rats after 30 min administration of Nimodipine (40 mg/kg) in ARR + Nimodipine group, and physiological saline was given to rats in the control and model groups. Thermal paw withdrawal latency and mechanical paw withdrawal reflex threshold of rats in each group were measured on one day before operation and on the days 1, 3, 5, 9, and 14 after 30 min of administration. Results The thermal paw withdrawal latency and mechanical paw withdrawal reflex threshold of rats in each group were not significantly different before and after the operation (P > 0.05). The thermal paw withdrawal latency and mechanical paw withdrawal reflex threshold of rats in the model group were significantly lower than those in the control group at various time points after the operation (P < 0.05 and 0.01). The thermal paw withdrawal latency and mechanical paw withdrawal reflex threshold of rats in ARR and Nimodipine groups were significantly higher than those in the model group from the day 5 after the operation (P < 0.05 and 0.01). The thermal paw withdrawal latency and mechanical paw withdrawal reflex threshold of rats in ARR + Nimodipine group were significantly higher than those in the model group from the day 3 after the operation (P < 0.05 and 0.01), and were significantly higher than those of both in ARR and Nimodipine groups from the day 5 after the operation (P < 0.05 and 0.01). Analgesic effects of ARR + Nimodipine group were better than those of separate ARR and Nimodipine groups. Conclusion The aqueous extract from ARR combined with Nimodipine has the ideal analgesic effects.