针刺“百会”、“大椎”对拟血管性痴呆大鼠脑内谷胱甘肽过氧化物酶及细胞凋亡的影响

目的 :建立拟血管性痴呆 (VD)的动物模型 ,探讨针刺“百会”及“大椎”对VD大鼠脑内谷胱甘肽过氧化物酶 (GSH PX)的影响并观察海马区内细胞凋亡情况。方法 :选用纯系Wistar老年大鼠 3 6只 ,随机分为假手术组、模型组、针刺组和西药组 ,采用 2 血管阻断法 ( 2 VO)制作VD大鼠模型 ,之后进行“百会”、“大椎”针刺治疗。检测大鼠穿梭箱实验成绩及脑内GSH PX的活力 ,采用TdT介导的原位末端标记法观察海马区内细胞凋亡情况。结果 :经统计学处理数据表明 ,针刺VD大鼠的“百会”、“大椎” ,可明显减少VD大鼠在穿梭箱实验中的电击次数和电击时间 ,增强脑内GSH PX的活力 ,明显减轻VD大鼠海马区内神经元的损伤。结论 :针刺“百会”、“大椎”能提高VD大鼠的学习记忆能力 ,改善自由基代谢 ,促进受损神经元的恢复。

Effect of Acupuncture of "Baihui" and "Dazhui" on the Activity of Cerebral GSH-PX and Neuronal Apoptosis in Vascular Dementia Rats

Objective:To observe the effect of acupuncture of “Baihui"(GV 20) and“Dazhui"(GV 14) on the activity of glutathione peroxidase (GSH PX) and the neuronal apoptosis in hippocampal region in vascular dementia rats. Methods:Thirty six Wistar aging rats were randomly and evenly divided into sham operation, model, acupuncture and medication groups. Vascular dementia model was established by occlusion of the bilateral common carotid arteries and reperfusion. In acupuncture group, “Baihui"(GV 20) and “Dazhui"(GV 14) were punctured with filiform needles and stimulated for 5?min by twirling the needles swiftly. In medication group, the rats were fed with Piracetum liquid (40 mg/mL, 6 mL/kg) via gastric tube. The treatment of two groups was conducted once daily, continuously for 30 days. GSH PX activity of the hippocampus was determined with TdT mediated dUTP biotin nick end labeling technique and apoptosis was detected with TUNEL method. Rats' behavior reaction was tested with active avoidance method (shuttle box). Results: The foot shock times and duration of model group were significantly more and longer than those of sham operation group ( P <0.001), while in comparison with model group, these two indexes of acupuncture and medication groups were significantly lower ( P <0.01). No significant differences were found between acupuncture and medication groups in these two indexes, suggesting that both acupuncture and medication can improve the memory of VD rats. In comparison with sham operation group, GSH PX activity decreased significantly ( P < 0 001 ); whereas compared with model group, GSH PX activity of both acupuncture and medication groups increased considerably ( P <0.01), and the effect of acupuncture group was significantly superior to that of medication group ( P <0.05). Results of HE staining indicated that in model group, there were a large number of apoptotic neurons in CA 1 area of the hippocampus, while the situation was apparently better in acupuncture and medication groups. Conclusion: Acupuncture of “Baihui" and “Dazhui" can apparently ameliorate the learning and memory ability of VD rats, raise GSH PX activity and reduce neuronal injury of the hippocampal region.