| 诱导型一氧化氮合酶与Th1/Th2型细胞因子在不明原因早期自然流产绒毛和蜕膜组织中的表达 |
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| 引用本文： | 夏革清,吴超英,孙永玉.诱导型一氧化氮合酶与Th1/Th2型细胞因子在不明原因早期自然流产绒毛和蜕膜组织中的表达[J].生殖医学杂志,2008,17(5):346-350. |
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| 作者姓名： | 夏革清 吴超英 孙永玉 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院妇产科,武汉,430022 |
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| 摘 要： | 目的了解不明原因早期自然流产局部绒毛、蜕膜组织中诱导型一氧化氮合酶(iNOS)和辅助性T细胞(Th1)/Th2型细胞因子的表达,进一步探讨不明原因早期自然流产发病机制。方法Th1/Th2型细胞因子分别以白细胞介素(IL)-2和IL-10为代表,采用原位杂交及免疫组化方法对40例自然流产患者绒毛和蜕膜中iNOS、IL-2、IL-10的表达(试验组)进行检测,利用计算机CMIAS系统,表达指标以阳性数密度(N/S)计算,并与40例正常人工流产的绒毛和蜕膜组织(对照组)比较。结果试验组iNOS、IL-2、IL-10原位杂交显示绒毛、蜕膜组织分别为(0.082±0.026)、(0.0026±0.0007)、(0.036±0.011)vs(0.10±0.03)、0、(0.02±0.006),对照组分别为(0.022±0.007)、(0.0028±0.0007)、(0.042±0.011)vs(0.03±0.009)、0、(0.032±0.006);两组比较iNOS、IL-10差异有显著意义(P<0.05)。免疫组化结果显示与原位杂交结果一致。结论INOS和ThI/Th2型细胞因子与自然流产相关,可能是介导流产发生的重要因素之一。
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| 关 键 词： | 诱导型一氧化氮合酶 细胞因子 流产 |
Expression of inducible nitric oxide synthase and Th1/Th2 cytokines in trophoblasts and deciduas in early spontaneous abortion |
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| XIA Ge-qing,WU Chao-ying,SUN Yong-yu.Expression of inducible nitric oxide synthase and Th1/Th2 cytokines in trophoblasts and deciduas in early spontaneous abortion[J].Journal of Reproductive Medicine,2008,17(5):346-350. |
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| Authors: | XIA Ge-qing WU Chao-ying SUN Yong-yu |
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| Institution: | XIA Ge-qing,WU Chao-ying,SUN Yong-yuDepartment of Obstetrics & Gynecology,Union Hospital of Huazhong University of Science & Technology,Wuhan 430022 |
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| Abstract: | Objective:To investigate the expression of inducible nitric oxide synthase(iNOS) and Th1/Th2 cytokines in chorionic villi and decidua in early spontaneous abortion,and to study the mechanism of spontaneous abortion.Methods:Th1/Th2 cytokines were represented by IL-2/IL-10.Expressions of iNOS,IL-2,IL-10 in chorionic villi and decidua were detected by both in situ hybridization and immunohistochemistry in 40 patients with spontaneous abortion(experiment group) and 40 normal controls.The expression was represen... |
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| Keywords: | iNOS Cytokine Spontaneous abortion |
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