| 大鼠FasL基因重组慢病毒载体介导大鼠肾脏转染的实验研究 |
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| 引用本文： | 吕清东,孙晓青.大鼠FasL基因重组慢病毒载体介导大鼠肾脏转染的实验研究[J].蚌埠医学院学报,2012,37(3):252-253. |
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| 作者姓名： | 吕清东 孙晓青 |
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| 作者单位： | 徐州市贾汪人民医院,泌尿外科,江苏,徐州,221011;徐州医学院附属医院,泌尿外科,江苏,徐州,221005 |
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| 摘 要： | 目的:使用FasL重组慢病毒载体通过大鼠肾动脉转染SD大鼠肾脏,观察转染后目的基因表达,为进一步进行移植实验奠定基础。方法:SD大鼠30只随机均分为实验组和对照组,实验组大鼠经肾动脉注入10 ng FasL重组慢病毒载体,对照组注入10 ng空白慢病毒载体,于转染后第3、7、15、25、40天分别采集标本,HE和IHC染色,观察组织形态、测定灰度值,并进行统计学分析。结果:各时段HE染色未见细胞形态改变及炎细胞浸润。IHC染色示转染后表达高峰出现于转染后第15天组。15天组与空白对照组差异有统计学意义(P0.05)。结论:大鼠FasL基因重组慢病毒载体成功转染大鼠肾脏并表达目的基因。
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| 关 键 词： | 慢病毒载体 转染 FasL |
The experimental study of the transfection recombinant lentiviral vector with FasL gene into rat kidney |
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| LU Qing-dong
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SUN Xiao-qing.The experimental study of the transfection recombinant lentiviral vector with FasL gene into rat kidney[J].Journal of Bengbu Medical College,2012,37(3):252-253. |
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| Authors: | LU Qing-dong SUN Xiao-qing |
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| Institution: | 2(Department of Urology,1.Jiawang People′s Hospital,Xuzhou Jiansu 221011; 2.The Affiliated Hospital of Xuzhou Medical College,Xuzhou Jiansu 221005,China) |
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| Abstract: | Objective:To explore the target gene expression of SD rat kidney transfected recombinant lentiviral vector with FasL and provide a basis of transplantation study.Methods:The recombinant lentiviral vector and empty vector(control) were injected into SD rat kidney through renal artery.The organization form and gray value of kidney tissues were detected by HE and immunohistochemistry(IHC) at days 3,7,15,25 and 40 after transfection and statistic analyzed.Results:No morphologic changes and inflammatory cells infiltration were found in kidney by HE staining..The peak of FasL gene expression was on the 15 day,which had significant difference compared with the control group(P<0.05).Conclusions:The recombinant lentiviral vector with FasL gene can be successfully transfected into SD rat kidney,which can express target gene. |
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| Keywords: | lentiviral vector transfect FasL |
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