| 用SMMC-7721和Hep-G2全二维细胞膜-整体柱色谱联用技术筛选中药鸦胆子的抗癌活性物质 |
| |
| 引用本文： | 纪松岗,丁璇,曹岩,陈啸飞,贾丹,王冬尧,柴逸峰. 用SMMC-7721和Hep-G2全二维细胞膜-整体柱色谱联用技术筛选中药鸦胆子的抗癌活性物质[J]. 药学实践杂志, 2014, 32(6): 425-427,433 |
| |
| 作者姓名： | 纪松岗 丁璇 曹岩 陈啸飞 贾丹 王冬尧 柴逸峰 |
| |
| 作者单位： | 1. 解放军401医院药剂科,山东青岛,266071
2. 第二军医大学药学院,上海,200433 |
| |
| 基金项目： | 国家自然科学基金青年项目(12401900802)． |
| |
| 摘 要： | 目的筛选中药鸦胆子中的抗癌活性成分。方法同时使用全二维色谱技术和细胞膜色谱技术联用质谱技术。结果经初步分离筛选,得到了腺苷和鸦胆子苦素B两种潜在的抗癌活性成分。结论该方法对中药中活性成分的筛选,具有快速、高效和在线的特色和优点。
|
| 关 键 词： | 细胞膜色谱 全二维色谱 SMMC-7721 Hep-G2 鸦胆子 抗癌 |
| 收稿时间： | 2014-09-04 |
| 修稿时间： | 2014-10-08 |
Screening of potential active anti-cancer components of Brucea javanica by SMMC-7721 and Hep-G2 comprehensive two dimensional CMC-monolith chromatography |
| |
| JI Songgang,DING Xuan,CAO Yan,CHEN Xiaofei,JIA Dan,WANG Dongyao and CHAI Yifeng. Screening of potential active anti-cancer components of Brucea javanica by SMMC-7721 and Hep-G2 comprehensive two dimensional CMC-monolith chromatography[J]. The Journal of Pharmaceutical Practice, 2014, 32(6): 425-427,433 |
| |
| Authors: | JI Songgang DING Xuan CAO Yan CHEN Xiaofei JIA Dan WANG Dongyao CHAI Yifeng |
| |
| Affiliation: | Department of Pharmacy, No.401 Hospital of PLA, Qingdao 266071, China,School of Pharmacy, Second Military Medical University, Shanghai 200433, China,School of Pharmacy, Second Military Medical University, Shanghai 200433, China,School of Pharmacy, Second Military Medical University, Shanghai 200433, China,School of Pharmacy, Second Military Medical University, Shanghai 200433, China,School of Pharmacy, Second Military Medical University, Shanghai 200433, China and School of Pharmacy, Second Military Medical University, Shanghai 200433, China |
| |
| Abstract: | Objective To screen potential active anti-cancer components of Brucea javanica.Methods This research has employed comprehensive two dimensional chromatographic technology and cell membrane chromatographic technology simultaneously with mass spectrometry as detector.Results Adenosine and Bruceine B were found to be potentially anti-cancer active.Conclusion This study has combined the advantages of online, high speed and high throughput for the screen of potential active components of traditional Chinese medicine. |
| |
| Keywords: | cell membrane chromatography comprehensive two dimensional chromatography SMMC-7721 Hep-G2 Bru-cea javanica anti-cancer |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《药学实践杂志》浏览原始摘要信息 |
|
点击此处可从《药学实践杂志》下载全文 |
|
