裂解血球全血与健康动物血清在牛多杀性巴士杆菌病灭活疫苗生产中使用效果对比试验

兽用生物制品制造及检验规程中规定:牛多杀性巴氏杆菌病灭活疫苗在通气培养过程中,需添加0.1%裂解血球全血。本试验通过在合成培养基中添加裂解血球全血与健康动物血清培养对比,发现在牛多杀性巴士杆菌病灭活疫苗生产中添加一定量健康动物血清,其生长菌数、效力检验、安全检验均与添加0.1%裂解血球全血生产检验指标相一致,且有降低污染率、降低生产成本的效果,值得生产中推广使用。

Compare the Effect of Whole Blood with Lysed Blood Cells and Healthy Animal Serum on the Production of Cattle Pasteurellamultocid Vaccine

Veterinary Biologics products industry and inspection procedures provided that: 0.1% whole blood with lysed blood cells was needed to add in aerobic culture of bovine pasteurellamultocida inactivated vaccinen.This test compare synthetic medium which was added whole blood with the medium with healthy animal serum-free culture,we found that there are consistent results in the production of cattle Pasteurellamultocid vaccine by adding a certain amount of healthy animal serum in cattle or 0.1% cleavage blood cell.And this method could lower pollution rate,reduce production costs,so it’s worth to use widely.