| 国产新华联屋尘螨点刺液生物活性的研究 |
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| 引用本文： | 祝戎飞,黄南,郭其苗,祁姗姗,张威,李文静,陈欢,刘光辉.国产新华联屋尘螨点刺液生物活性的研究[J].华中科技大学学报(医学版),2011,40(3):361-364. |
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| 作者姓名： | 祝戎飞 黄南 郭其苗 祁姗姗 张威 李文静 陈欢 刘光辉 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院过敏反应科,武汉,430030 |
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| 摘 要： | 目的探讨国产新华联屋尘螨点刺液的体内和体外生物活性。方法收集60例单纯尘螨过敏的哮喘患者血清,制备屋尘螨血清池,采用放射过敏原免疫吸附(RAST)抑制试验方法测定新华联屋尘螨点刺液体外生物活性并与德国阿罗格屋尘螨点刺液和丹麦ALK屋尘螨点刺液进行比较;选择90例疑诊过敏性疾病患者,采用新华联屋尘螨点刺液和阿罗格屋尘螨点刺液进行皮肤点刺试验,比较其体内生物活性。采用聚丙烯酰胺凝胶电泳法比较不同屋尘螨点刺液蛋白组分有无差异;酶联免疫吸附法(ELISA)测定屋尘螨点刺液主要致敏蛋白Der p1的含量。结果 RAST抑制试验显示3批次的新华联屋尘螨点刺液均能抑制标准屋尘螨抗原与IgE的特异性结合,其体外生物活性与阿罗格和ALK屋尘螨点刺液相似;皮肤点刺试验显示新华联屋尘螨点刺液的体内生物活性与阿罗格屋尘螨点刺液相似;电泳结果提示3批次的新华联屋尘螨点刺液蛋白组分相同,与阿罗格和ALK屋尘螨点刺液组分相似;ELISA法检测发现3批次的新华联屋尘螨点刺液主要致敏蛋白Der p1含量相同,低于ALK屋尘螨点刺液,但高于阿罗格屋尘螨点刺液。结论国产新华联屋尘螨点刺液具有较强的体内和体外生物活性,可用于屋尘螨过敏的诊断。
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| 关 键 词： | 屋尘螨 皮肤点刺试验 放射免疫吸附抑制试验 生物活性 |
Bioactivity of Commercial Chinese Xinhualian Dermatophagoides Pteronyssinus Skin Prick Solution in vivo and in vitro |
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| Zhu Rongfei,Huang Nan,Guo Qimiao et al.Bioactivity of Commercial Chinese Xinhualian Dermatophagoides Pteronyssinus Skin Prick Solution in vivo and in vitro[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(3):361-364. |
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| Authors: | Zhu Rongfei Huang Nan Guo Qimiao |
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| Institution: | Zhu Rongfei,Huang Nan,Guo Qimiao et alDepartment of Allergy,Tongji Hospital,Tongji Medical College,HuazhongUniversity of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective To investigate the bioactivity of commercial Chinese Dermatophagoides pteronyssinus(Dp)skin prick solution in vivo and in vitro.Methods The sera from 60 patients with dust-mite allergic asthma were collected to prepare an allergic sera pool.Radioallergosorbent test(RAST)inhibition was performed to test bioactivity of Xinhualian,Allergopharma and ALK skin prick solution respectively in vitro.Skin prick tests were performed on 90 patients suspected of having allergic diseases with both Xinhualian an... |
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| Keywords: | Dermatophagoides pteronyssinus skin prick test RAST inhibition bioactivity |
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