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| 分泌性卷曲相关蛋白3影响小鼠胚胎肝干细胞体外成熟分化的实验研究 | |
| 引用本文： | 贺引,蒋昌科,何昀,冯涛,毕杨. 分泌性卷曲相关蛋白3影响小鼠胚胎肝干细胞体外成熟分化的实验研究[J]. 华中科技大学学报(医学版), 2011, 40(3): 268-273. DOI: 10.3870/j.issn.1672-0741.2011.03.005 |
| 作者姓名： | 贺引 蒋昌科 何昀 冯涛 毕杨 |
| 作者单位： | 1. 重庆医科大学附属儿童医院儿科研究所干细胞实验室,重庆,400014;重庆市第九人民医院儿科,重庆,400700 2. 重庆医科大学附属儿童医院儿科研究所干细胞实验室,重庆,400014;重庆医科大学附属永川医院儿科,重庆,402160 3. 重庆医科大学附属儿童医院儿科研究所干细胞实验室,重庆,400014 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的 检测不同发育阶段的肝组织中Wnt信号拮抗因子分泌性卷曲相关蛋白(secreted frizzled-related protein,SFRP)的表达,并探讨SFRP3对胚胎肝干细胞体外成熟分化的影响.方法 分离胚胎12.5 d至出生后4周共9个阶段的小鼠肝组织,RT-PCR检测5种SFRPs在肝组织及胚胎肝干细胞... |
| 关 键 词： | 胚胎肝干细胞 分化抑制 分泌性卷曲相关蛋白3 Wnt信号拮抗因子 |
Effect of Secreted Frizzled-related Protein 3 on Differentiation of Embryonic Liver Stem Cells in vitro |
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| He Yin,,Jiang Changke,,He Yun et alStem Cell Biology , Therapy Laboratory,The Children's Hospital of Chongqing Medical University,Chongqing ,China. Effect of Secreted Frizzled-related Protein 3 on Differentiation of Embryonic Liver Stem Cells in vitro[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2011, 40(3): 268-273. DOI: 10.3870/j.issn.1672-0741.2011.03.005 | |
| Authors: | He Yin Jiang Changke He Yun et alStem Cell Biology Therapy Laboratory The Children's Hospital of Chongqing Medical University Chongqing China |
| Affiliation: | He Yin1,2,Jiang Changke1,3,He Yun1 et al1Stem Cell Biology and Therapy Laboratory,The Children's Hospital of Chongqing Medical University,Chongqing 400014,China2Department of Pediatrics,Chongqing Ninth People's Hospital,Chongqing 400700,China3Department of Pediatrics,Yongchuan Affiliated Hospital of Chongqing Medical University,Chongqing 402160,China |
| Abstract: | Objective To detect the expression levels of Wnt antagonists,secreted frizzled-related proteins(SFRPs),in different developmental stages of liver tissues,and investigate the effect of SFRP3 on the differentiation of embryonic liver stem cells in vitro.Methods Liver tissues were isolated from embryos 12.5 days to postnatal 4 weeks in mice.The expression profiles of 5 SFPRs in liver tissue and ELSC14.5 cells were detected by RT-PCR.ELSC14.5 cells were infected with adenovirus SFRP3,and treated with dexamethas... |
| Keywords: | embryonic liver stem cells inhibition of differentiation secreted frizzled-related protein 3 Wnt antagonist |
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