| 应用iTRAQ标记结合2D LC-MS/MS分析慢性苯中毒患者血清蛋白质组 |
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| 引用本文： | 黄振烈,王海兰,黄汉林,夏丽华,邱新香,陈慈珊,陈嘉斌,陈甦生,梁伟辉,黄明,郎丽,郭义曹. 应用iTRAQ标记结合2D LC-MS/MS分析慢性苯中毒患者血清蛋白质组[J]. 中国职业医学, 2011, 38(5) |
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| 作者姓名： | 黄振烈 王海兰 黄汉林 夏丽华 邱新香 陈慈珊 陈嘉斌 陈甦生 梁伟辉 黄明 郎丽 郭义曹 |
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| 作者单位： | 广东省职业病防治院,广东广州,510300 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金，广东省医学科学技术研究基金 |
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| 摘 要： | 目的 比较慢性苯中毒患者与健康人的血清蛋白质组,筛选差异表达蛋白质,为阐明其发病机制和寻找生物标志物提供靶标.方法 免疫层析柱去除14种高丰度血清蛋白质后,应用8标iTRAQ技术结合2D LC-MS/MS分析并鉴定慢性苯中毒病例与健康人血清的差异表达蛋白质.结果 对9例慢性苯中毒患者和9名健康人的血清蛋白样品的iTRAQ标记,分析并成功鉴定159种蛋白质,其中差异表达有统计学意义(P＜0.05)的蛋白质有纤维蛋白溶酶原(PLG)、载脂蛋白B100( APOB100)、血小板碱性蛋白(PBP).这些差异表达蛋白质具有结合、催化、酶调节和转运活性等分子功能,参与的生物过程包括免疫、代谢、应激、转运和凋亡等.结论 PLG、APOB100和PBP可能作为慢性苯中毒发病机制研究的候选靶标.
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| 关 键 词： | 苯 iTRAQ 2D LC-MS/MS 血清 |
Quantitative proteomic analysis on serum in patients with chronic benzene poisoning using 8-plex iTRAO labeling coupled with 2D LC-MS/MS |
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| HUANG Zhen-lie,WANG Hai-lan,HUANG Han-lin,XIA Li-hua,OIU Xin-xiang,CHEN Ci-shan,CHEN Jia-bin,CHEN Su-sheng,LIANG Wei-hui,HUANG Ming,LANG Li,GUO Yi-cao. Quantitative proteomic analysis on serum in patients with chronic benzene poisoning using 8-plex iTRAO labeling coupled with 2D LC-MS/MS[J]. China Occupational Medicine, 2011, 38(5) |
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| Authors: | HUANG Zhen-lie WANG Hai-lan HUANG Han-lin XIA Li-hua OIU Xin-xiang CHEN Ci-shan CHEN Jia-bin CHEN Su-sheng LIANG Wei-hui HUANG Ming LANG Li GUO Yi-cao |
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| Affiliation: | HUANG Zhen-lie,WANG Hai-lan,HUANG Han-lin,XIA Li-hua,QIU Xin-xiang,CHEN Ci-shan,CHEN Jia-bin,CHEN Su-sheng,LIANG Wei-hui,HUANG Ming,LANG Li,GUO Yi-cao(Guangdong Prevention and Treatment Center for Occupational Diseases,Guangzhou 510300,China) |
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| Abstract: | Objective To compare the expression of serum proteins between patients with chronic benzene poisoning and healthy controls,and to screen the differentially expressed proteins for the study of molecular mechanisms and the development of biomarkers for treatment and prevention. Methods 14 kinds of high abundant proteins in the serum were removed by immunochromatography assay.Isobaric tags for relative and absolute quantitation(iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry(... |
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| Keywords: | Benzene iTRAQ 2D LC-MS/MS Serum |
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