不同剂量刀豆蛋白A诱导Balb/c小鼠肝纤维化的量效关系

目的观察不同剂量刀豆蛋白A（Con A）诱导小鼠肝纤维化的效果,探讨建立小鼠肝纤维化动物模型所需最佳剂量及成模时间。方法实验组Balb/c小鼠18只,再分为A组、B组、C组,每组6只,经尾静脉分别注射10 mg/kg、12.5 mg/kg、15 mg/kg的Con A,每周1次。对照组6只,经尾静脉注射等量PBS,每周1次。各组均于每次注射后24 h采血,测丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）;于实验第5、7、9周,每组各处死2只小鼠,取血清检测血清透明质酸,留肝脏作病理检查。结果A组第9周、B组第7周出现肝纤维化,C组第5周出现肝纤维化,但死亡2只。实验组ALT、AST、明显高于对照组（P〈0.05）。结论12.5 mg/kg Con A经尾静脉注射小鼠是合适的剂量,每周1次,注射6次可建立肝纤维化动物模型。

A study on the dose-effect relationship between the dosage of concanavalin A and hepatic fibrosis in Balb/c mice

Objective To optimize the dosage of concanavalin A(Con A) to establish animal model of hepatic fibrosis in Balb/c mice.Methods 24 Balb/c mice were divided into 1 control group and A,B and C,3 model groups(n=6 each).The model groups were given Con A 10 mg/kg,12.5 mg/kg and 15 mg/kg iv once a week,respectively,with the control group given PBS.Serum was collected 24 h after injection to determine the levels of ALT and AST.2 mice in each group were killed at the 5th,7th and 9th week to measure the HA content and to proceed with the pathology of liver tissue.Results Hepatic fibrosis was induced at 9th week in group A,at 7th week in group B,at 5th week in group C,but 2 mice in the last group died of the toxic effects of Con A.Conclusion Con A 12.5 mg/kg iv once a week for 6 weeks is an acceptable protocol for inducing mouse model of hepatic fibrosis.