| 培养基组分对蛹虫草生物量及核苷、碱基积累的影响 |
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| 引用本文： | 顾宇翔,王尊生,李素霞,袁勤生.培养基组分对蛹虫草生物量及核苷、碱基积累的影响[J].中国医药工业杂志,2006,37(10):661-664. |
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| 作者姓名： | 顾宇翔 王尊生 李素霞 袁勤生 |
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| 作者单位： | 1. 华东理工大学生物反应器国家重点实验室,上海,200237
2. 华东理工大学生物反应器国家重点实验室,上海,200237;沈阳师范大学生物系,辽宁,沈阳,110034 |
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| 基金项目： | 上海市重点学科建设项目 |
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| 摘 要： | 考察了碳源、氮源和金属离子对蛹虫草发酵生物量和7种核苷、碱基积累的影响，结果表明以20％土豆汁＋2％葡萄糖为碳源，0.3％酵母膏＋0.3％蛋白胨为氮源，并在培养基中加入0．1mmol／LMn^2＋能促进菌体生长和核苷、碱基的积累。在最佳条件下，腺苷和3＇-脱氧腺苷在菌丝体中含量分别为4．96、0．199mg／g，在气生菌丝体中分别为4．91、7．22mg／g。
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| 关 键 词： | 蛹虫草 发酵 核苷 碱基 |
| 文章编号： | 1001-8255（2006）10-0661-04 |
| 收稿时间： | 2005-09-12 |
| 修稿时间： | 2005-09-12 |
Effects of Medium Compositions on the Biomass and Accumulation of Nucleosides and Bases in Cordyceps militaris |
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| Authors: | GU Yu-Xiang WANG Zun-Sheng LI Su-Xia YUAN Qin-Sheng |
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| Institution: | 1. State Key Lab. of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237;2. Dept.of Biology, Shenyang Normal University, Shenyang 110034 |
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| Abstract: | The effects of medium compositions including carbon sources, nitrogen sources and metal ions on both Cordyceps militaris biomass and the contents of nucleosides and bases were investigated. The results showed that the strain growth and the production of nucleosides and bases could be promoted by use of complex carbon sources (20% potato extract adding 2% glucose) and complex nitrogen sources (0.3% yeast extract and 0.3% peptone) with 0.01mol/L Mn2+ in the medium. Under the best fermentation conditions, the contents of adenosine and 3'-deoxyadenosine in mycelia were 4.96 and 0.199mg/g, while 4.91 and 7.22mg/g in aerial mycelia , respectively. |
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| Keywords: | Cordyceps militaris fermentation nucleoside base |
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