端粒酶在宫颈癌中的表达及意义

目的　探讨端粒酶与宫颈癌及与淋巴结转移的关系。方法　采用银染色端粒酶重复扩增法(TRAP)检测各类宫颈组织中端粒酶活性。结果　正常宫颈、慢性宫颈炎、宫颈上皮内瘤样病变组织端粒酶的阳性表达率分别为 6 .6 7%、8.5 7%、2 6 .32 % ,与宫颈癌组织阳性表达率 (78.85 % )比较具有非常显著性差异 (P <0 .0 1) ;宫颈癌组织无或有淋巴结转移其端粒酶活性阳性表达率分别为 6 9.70 %、94 .74 % ,两者之间比较亦有显著性差异 (P <0 .0 5 )。结论　端粒酶活性在宫颈癌组织中增高且与淋巴结转移相关

The expression and significance of tolermerase enzyme in cervical carcinoma

Objective To study the relation between tolermerase enzyme and cervical carcinoma and lymphatic metastasis.Methods The activity of tolermerase enzyme was detected by silver staining tolermerase enzyme TRAP.Results The positive expression rates of tolermerase enzyme for normal cervical tissue,chronic cervicitis and cervical intraepithelial neoplasia were respectively 6.67 %,8.57 % and 26.32 %,and there was significant difference( P <0.01) in comparison with that of cervical carcinoma tissue (78.85 %); the positive expression rates of tolermerase enzyme for cervical intraepithelial neoplasia or no cervical intraepithelial neoplasia were repectively 69.70 % and 94.74 % and there was significant difference( P <0.05) between them.Conclusion The activity of tolermerase enzyme in cervical carcinoma tissue increases and is related to lymphatic metastasis.