附子对腺嘌呤造成的小鼠慢性肾衰的疗效及其机制探讨

目的:研究中药附子对腺嘌呤造成的小鼠慢性肾衰的疗效,并探讨其作用机制。方法:选取雄性小鼠随机分为4组:对照组(C),模型组(M),附子低剂量组(FL),附子高剂量组(FH)。对照组给予溶剂,模型组灌胃给予腺嘌呤,各给药组在给予腺嘌呤的基础上,再分别给予3g/kg/d、10g/kg/d的附子水煎剂。实验结束时,测定血清尿素氮、肌酐、γ-谷氨酰转肽酶,肾组织γ-谷氨酰转肽酶、一氧化氮、谷胱甘肽、自由基等的含量,并记录附睾精子数、全血血红蛋白含量。结果:附子高剂量组优于低剂量组,能降低尿素氮、肾系数,提高精子数、血红蛋白含量。而附子低剂量组则无明显疗效。结论:附子高剂量显示对腺嘌呤造成的慢性肾衰有一定的疗效,其作用机制还有待于进一步研究。

The Effects and Mechanism of Fuzi on Chronic Renal Failure in Mice

Objective： To observe the effect and mechanism of Fuzi on chronic renal failure in mice.Methods： Male mice were randomly divided into four groups,that is,normal control group,model group（adenine,250 mg/kg every two days in the first week,130mg/kg every two days in the second and third weeks）,Fuzi high dose group（10g/kg/d）,Fuzi low dose group（3g/kg/d）.At the end of the experiment,we measured the content of blood urea nitrogen（BUN）,creatine（Cr）,γ-glutamyltranspeptidase（γ-GT） in blood serum.The kidney were homogenated for measuring γ-GT,nitric oxide（NO） and glutathione（GSH）.Besides,we recorded the number of sperm in the left epididymis and the content of hemoglobin in blood.Results： the high dose Fuzi could reduce the blood urea nitrogen,renal factor,increase sperm count,hemoglobin content,while the low dose Fuzi could not.Conclusions： Fuzi high dose group showed some efficacy,the mechanism needs to be studied further.