| 一步法分析细胞色素P450 2D6酶活性缺陷等位基因CYP2D6A和CYP2D6B |
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| 引用本文： | 陈枢青 Wecll.,P. 一步法分析细胞色素P450 2D6酶活性缺陷等位基因CYP2D6A和CYP2D6B[J]. 中国药学杂志, 1998, 33(10): 614-616 |
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| 作者姓名： | 陈枢青 Wecll. P |
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| 作者单位： | 浙江医科大学生物化学教研室 杭州 310006; 1美国肯塔基大学药学院 |
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| 基金项目： | 本文部分工作得到浙江省医药卫生科学研究基金的资助，主要工作在美国肯塔基大学药学院Dr.Wedlund实验室完成 |
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| 摘 要： | 目的：CYP2D6A和CYP2D6B是引起细胞色素P4502D6(CYP2D6)酶活性缺陷的最主要的等位基因 ，对CYP2D6A和CYP2D6B的检测可准确(>92%)预测CYP2D6慢代谢者。本研究利用等位基因特异扩增法，建立了一步PCR法测定CYP2D6A和CYP2D6B等位基因。方法：等位基因特异扩增法分析CYP2D6A和CYP2D6B等位基因；右美沙芬作为探针药物测定表型。结果：经130例测定，说明本法更为快捷、更少污染。结论：本法的建立为该项测定应用于临床、指导临床合理用药奠定基础。
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| 关 键 词： | 细胞色素P4502D6(CYP2D6) 基因分型 CYP2D6A CYP2D6B 等位基因特异扩增法 |
| 收稿时间： | 1997-07-28; |
Genotyping of cytochrome P450 2D6 enzyme deficient alleles CYP2D6A and CYP2D6B by one-step ASA analysis |
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| Chen Shuqing. Genotyping of cytochrome P450 2D6 enzyme deficient alleles CYP2D6A and CYP2D6B by one-step ASA analysis[J]. Chinese Pharmaceutical Journal, 1998, 33(10): 614-616 |
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| Authors: | Chen Shuqing |
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| Affiliation: | (Chen SQ)，Peter J Wedlun |
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| Abstract: | OBJECTIVE: Cytochrome P450 2D6 enzyme deficiency is mainly caused by CYP2D6A and CYP2D6B alleles. The determination of the alleles could predict CYP2D6 poor metabolizers with an accuracy of more than 92%. This study was to build up one-step PCR assay to analyze CYP2D6A and CYP2D6B alleles. METHOD: The principle of allele-specific amplification (ASA) was used to set up one-step PCR analysis and dextromethorphan was used as probe drug to phenotype subjects. RESULTS: The method was proved to be efficient and less contaminated in 130 applications. CONCLUSION: It provides a basis for the clinical use of this determination. |
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| Keywords: | cytochrome P4502D6 genotyping CYP2D6A CYP2D6B allele-specific amplification |
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