| 用PCR技术检测皮肤表皮肿瘤组织HPV DNA |
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| 引用本文： | 刘跃华,王家璧.用PCR技术检测皮肤表皮肿瘤组织HPV DNA[J].中国医学科学院学报,1997,19(1):64-66. |
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| 作者姓名： | 刘跃华 王家璧 |
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| 作者单位： | 中国医学科学院中国协和医科大学协和医院!北京，100730，中国医学科学院中国协和医科大学协和医院!北京，100730，中国医学科学院中国协和医科大学协和医院!北京，100730，中国医学科学院中国协和医科大学协和医院!北京，100730 |
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| 摘 要： | 应用多对引物ＰＣＲ方法检测５８例皮肤人表皮肿瘤（Ｂｏｗｅｎ‘ｓ病１７例，Ｑｕｅｙｒａｔ增殖性红斑５例，鲍温样丘疹病１６例，皮肤鳞状细胞癌２０例）石蜡包埋组织中ＨＰＶ６，１１，１６及１８ＤＮＡ。Ｂｏｗｎｅ’ｓ病ＨＰＶ１ＤＮＡ２例（２／１７）阳性Ｑｕｅｙｒａｔ增殖性红斑ＨＰＶ１６ＤＮＡ１例（１／５）阳性，鲍温样丘疹病ＨＰＶ１６ＤＮＡ９例（９／１６）阳性，皮阳鳞状细胞癌ＨＰＶＤＮＡ２０例均阴性，提示鲍温
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| 关 键 词： | 人乳头状瘤病毒 表皮肿瘤 聚合酶链反应 |
Detecting HPV DNA in Tissue of Epidermal Neoplasms |
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| Liu Yuehua, Wang Jiabi, Wang Xiaofeng, Fang Kai.Detecting HPV DNA in Tissue of Epidermal Neoplasms[J].Acta Academiae Medicinae Sinicae,1997,19(1):64-66. |
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| Authors: | Liu Yuehua Wang Jiabi Wang Xiaofeng Fang Kai |
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| Institution: | PUMC Hospital, Beijing. |
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| Abstract: | Fifty-eight cases (mean age 56.1 +/- 17.1 years) of cutaneous squamous cell carcinoma were studied, including 17 cases of Bowen's disease, 5 cases of Queyrat erythroplasia, 16 cases of Bowenoid papulosis and 20 cases of invasive cutaneous squamous cell carcinoma. All cases were confirmed by pathological findings. HPV6, 11, 16, 18 DNA were detected by multiple pair primers polymerase chain reaction in paraffin-embedded tissues. The results showed that 2(2/17) cases of Bowen's disease, 1 case of Queyrat erythroplasia and 9(9/16) cases of Bowenoid papulosis were positive for HPV16 DNA and that 20 cases of invasive cutaneous sqamous cell carcinoma were negative for HPV16 DNA. HPV16 is closely associated with Bowenoid papulosis and HPV16 might be a factor of multistep carcinogenesis in rare cases of Bowen's disease and Queyrat erythroplasia. |
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| Keywords: | human papillomavirus epidermal neoplasm bowenoid papulosis Bowen's disease |
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