| 免疫亲和毛细管电泳技术的进展 |
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| 引用本文： | 罗国安,温涛,王义明,王清刚. 免疫亲和毛细管电泳技术的进展[J]. 药学学报, 2002, 37(6): 479-484 |
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| 作者姓名： | 罗国安 温涛 王义明 王清刚 |
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| 作者单位： | 清华大学化学系,北京,100084 |
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| 基金项目： | SupportedbytheNationalNatureScienceFoundationofChina (2 963 5 0 2 0 ) . |
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| 摘 要： | 1 ＩｎｔｒｏｄｕｃｔｉｏｎＩｍｍｕｎｏａｓｓａｙ (ＩＡ ) ,ｏｎｅｏｆｔｈｅｍｏｓｔｕｓｅｆｕｌｂｉｏｍｅｄｉｃａｌｔｏｏｌｓｉｎｔｈｅｌａｓｔｆｏｕｒｄｅｃａｄｅｓ[1～ 3 ] ,ｉｓａｍｅｔｈｏｄｂａｓｅｄｏｎｔｈｅｓｐｅｃｉｆｉｃｉｎｔｅｒａｃｔｉｏｎｓｂｅｔｗｅｅｎａｎｔｉｂｏｄｉｅｓａｎｄａｎｔｉｇｅｎｓ,ａｎｄｉｓｕｓｅｄｔｏｄｅｔｅｒｍｉｎｅｄｉｆｆｅｒｅｎｔａｎｔｉｇｅｎｓ ,ｈａｐｔｅｎｓ,ｏｒａｎｔｉｂｏｄｉｅｓｉｎｃｏｍｐｌｅｘｂｉｏｌｏｇｉｃａｌｍａｔｒｉｃｅｓｂｙｔａｋｉｎｇａｎ…
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| 关 键 词： | 免疫亲和毛细管电泳 竞争性免疫 夹心免疫 富集 亲和 |
| 收稿时间： | 2001-06-08 |
ADVANCE OF IMMUNOAFFINITY CAPILLARY ELECTROPHORESIS |
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| LUO Guo-an,WEN Tao,WANG Yi-ming,WANG Qing-gang. ADVANCE OF IMMUNOAFFINITY CAPILLARY ELECTROPHORESIS[J]. Acta pharmaceutica Sinica, 2002, 37(6): 479-484 |
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| Authors: | LUO Guo-an WEN Tao WANG Yi-ming WANG Qing-gang |
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| Affiliation: | Department of Chemistry, Tsinghua University, Beijing 100084, China. luoga@chem.tsinghua.edu.cn |
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| Abstract: | 1 Introduction Immunoassay (IA), one of the most useful biomedical tools in the last four decades[1～3], is a method based on the specific interactions between antibodies and antigens, and is used to determine different antigens, haptens, or antibodies in complex biological matrices by taking antigens or antibodies as selective agents. Conventional immunoassays can be further classified into labeling techniques as radioactivity, enzyme amplification, fluorescence and chemiluminescence[4]. |
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| Keywords: | competitive immunoassay sandwich immunoassay pre-concentration affinity immunoaffinity capillary electrophoresis |
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