| 兔体内乌头碱代谢产物研究 |
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| 引用本文： | 孙莹,张宏桂,史向国,段明郁,钟大放.兔体内乌头碱代谢产物研究[J].药学学报,2002,37(10):781-783. |
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| 作者姓名： | 孙莹 张宏桂 史向国 段明郁 钟大放 |
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| 作者单位： | 1. 吉林大学药学院天然药物化学教研室,吉林,长春,130021
2. 沈阳药科大学药物代谢与药物动力学实验室,辽宁,沈阳,110016 |
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| 基金项目： | 国家自然科学基金资助项目 (3 9870 874，3 993 0 180 ) . |
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| 摘 要： | 目的鉴定乌头碱在家兔尿中主要代谢产物。方法ig给药(5 mg·kg-1)后,收集给药后♂家兔尿液,固相萃取柱富集、液相色谱-电喷雾离子阱质谱法分离鉴定乌头碱代谢物。结果在给药后尿样中发现乌头碱原形和4种代谢物M1～M4,分别测得准分子离子峰［M+H］+及其各级碎片离子。结论M1～M4为乌头碱的4个代谢物,推测分别为16-O-去甲基乌头碱、乌头次碱、16-O-去甲基乌头次碱、乌头原碱。
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| 关 键 词： | 乌头碱 代谢物 液相色谱-电喷雾离子阱质谱法 |
| 收稿时间： | 2001-09-28 |
STUDY ON METABOLITES OF ACONITINE IN RABBIT URINE |
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| SUN Ying,ZHANG Hong-gui,SHI Xiang-guo,DUAN Ming-yu,ZHONG Da-fang.STUDY ON METABOLITES OF ACONITINE IN RABBIT URINE[J].Acta Pharmaceutica Sinica,2002,37(10):781-783. |
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| Authors: | SUN Ying ZHANG Hong-gui SHI Xiang-guo DUAN Ming-yu ZHONG Da-fang |
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| Institution: | Department of Natural Pharmaceutical Chemistry, School of Pharmacy, jilin University, Changchun 130021, China. |
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| Abstract: | AIM: To identify the main metabolites of aconitine in the urine of rabbits. METHODS: After oral administration of aconitine (5 mg.kg-1), the urine of male rabbits was collected and extracted by solid phase extraction and analyzed by liquid chromatography-ion trap mass spectrometry. RESULTS: Aconitine and 4 metabolites were found in the rabbit urine. Their protonated molecular ions at m/z 632, m/z 604, m/z 590, m/z 500 and multistage fragment ions with neutral loss of 60 u, 32 u, 28 u and 18 u were monitored. Their relative concentration were M1 > Aconitine > M4 > M2 > M3. CONCLUSION: The metabolites M1-M4 were deduced as 16-O-demethylaconitine, benzoylaconine, 16-O-demethylbenzoylaconine and aconine, respectively. |
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| Keywords: | aconitine metabolite liquid chromatography ion trap mass spectrometry |
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