2-乙基-3-羟基-6-苯硫基-4(1H)-吡啶酮的抗氧化作用

目的研究2-乙基-3-羟基6-苯硫基-4(1H)-吡啶酮(HPP)的抗氧化作用。方法用电子自旋共振(ESR)法测定HPP对羟自由基的清除作用,用荧光法测定大鼠线粒体膜的流动性,用比色法测定给药后小鼠血清中超氧化物歧化酶(SOD)及肝线粒体中谷胱甘肽过氧化物酶(GSH-Px)的活力。结果HPP对羟自由基有较强的清除作用,显著促进线粒体膜的流动性,明显提高给药小鼠血清中SOD和线粒体中GSH-Px的活力。结论HPP有显著的抗氧化作用。

ANTIOXIDANT ACTIVITY OF 2-ETHYL-3-HYDROXY-6- PHENYLTHIO-4(1H)-PYRIDONE

AIMTo study the antioxidant activity of 2-ethyl-3-hydroxy-6-phenylthio-4(1H)-pyridone (HPP). METHODSThe scavenging effect of HPP on hydroxyl radical was determined using electron spin resonance (ESR), DMPO was used as free radical capture and the fluidity of the mitochondria membrane of mouse liver was measured using fluorescence spectrophotometer, DPH was used as fluorescence probe. The mice were injected ip with HPP 0.1, 0.4 and 1.6 mg·kg^-1 once daily for two weeks. The serum and the liver mitochondria of the tested mice were separated to quantify the liver GSH-Px and the serum SOD activities through colorimetric reaction. MDA was assayed by TBA color reaction. RESULTSIt is evident that HPP considerably scavenged hydroxyl radical and significantly increased the fluidity of the mouse liver mitochondria membrane. HPP greatly enhanced the serum SOD activity and the liver mitochondria GSH-Px activity while the MDA levels in mouse liver tissues decreased. CONCLUSIONThe antioxidant activity of HPP is evident.