硫酸镁对大鼠海马神经元瞬间外向钾电流和延迟整流钾电流的抑制作用

目的研究硫酸镁(MgSO₄)对大鼠海马神经元瞬间外向钾电流(I_A)和延迟整流钾电流(I_K)的作用。方法 全细胞膜片钳技术。结果MgSO₄可浓度依赖地抑制I_A和I_K,半数抑制浓度IC₅₀分别为6.30和7.60 mmol·L^-1;此外还与电压呈依赖性关系,但不具有频率依赖性。6 mmol·L^-1 MgSO₄非常显著地影响I_A和I_K的激活过程,但不改变二者的斜率因子。另外6 mmol·L^-1 MgSO₄还非常显著地影响I_A的失活过程,但不改变其斜率因子。结论 MgSO₄抑制大鼠海马神经元的I_A和I_K,并改变I_A和I_K的激活过程和失活过程。这可能是其抗缺血缺氧引发的中枢神经系统损伤,具有神经保护作用的机制之一。

BLOCKADE OF MAGNESIUM SULFATE ON TRANSIENT OUTWARD K+ CURRENT AND DELAYED RECTIFIER K+ CURRENT IN ACUTELY ISOLATED RAT HIPPOCAMPAL NEURONS

AIM: To study the effect of magnesium sulfate on transient outward K+ current (IA) and delayed rectifier K+ current (IK) in freshly dissociated hippocampal neurons of rats. METHODS: The whole-cell patch clamp techniques were used. RESULTS: Magnesium sulfate reversibly reduced the amplitudes of IA and IK in a concentration-dependent and voltage-dependent, but not frequency-dependent manner. Half-blocking concentration (IC50) on IA and IK were 6.30 mmol.L-1 and 7.60 mmol.L-1, respectively. Magnesium sulfate (6 mmol.L-1) affected the activation process of IA and IK. Before and after application of the drug, the half-activation voltages of IA were (7 +/- 6) mV and (-7 +/- 11) mV (n = 10, P < 0.01), and the half-activation voltages of IK were (20 +/- 6) mV and (28 +/- 4) mV (n = 10, P < 0.01), but the slope factors were not changed. In addition, magnesium sulfate (6 mmol.L-1) also affected the inactivation process of IA. Before and after application of the drug, the half-inactivation voltages of IA were (-65 +/- 5) mV and (-89 +/- 6) mV (n = 10, P < 0.01). CONCLUSION: Magnesium sulfate inhibited IA and IK in freshly dissociated hippocampal neurons of rats, which might contribute to protect the central neuronal system (CNS) against damages induced by ischemia and oxygen deprivation.