姜黄素拮抗亚砷酸钠所致的DNA链断裂损伤

目的观察姜黄素对亚砷酸钠诱发小鼠体内免疫器官细胞DNA链断裂损伤的拮抗作用。方法用单细胞凝胶电泳法。结果亚砷酸钠单独染毒后的DNA链断裂损伤的高峰期各器官不同,脾脏为染毒后1 h,胸腺和骨髓在染毒后3 h。姜黄素拮抗亚砷酸钠引起的各器官DNA链断裂损伤的作用在高峰期明显,表现为彗星细胞百分比降低,并有量效关系。结论姜黄素可以预防亚砷酸钠诱发的小鼠脾脏、胸腺和骨髓细胞的DNA链断裂损伤。

ANTAGONISTIC EFFECT OF CURCUMIN ON SODIUM ARSENITE INDUCED DNA DAMAGE IN MICE

AIMTo investigate the antagonistic effect of curcumin on DNA damage in immune organs in vivo induced by sodium arsenite (NaAsO₂). METHODSMale ICR mice were given curcumin 2, 20 and 200 mg·kg^-1 for 14 days before treatment with sc injection of NaAsO₂ at the dose of 10 mg·kg^-1. After injection, the mice were sacrificed at the end of 1 and 3 hours. DNA strand breaks were detected in the splenocyte, thymocyte and marrow cells through single cell gel electrophoresis (SCGE). RESULTSCurcumin was shown to inhibit DNA strand breaks induced by NaAsO₂ in a dose-dependent manner. It was found that curcumin 20 and 200 mg·kg^-1 could reduce the DNA strand breaks induced by NaAsO₂ 1 hour after treatment in cells from spleen. The percent of “comet” cell was decreased. Curcumin 20 and 200 mg·kg^-1 treatment was shown to reduce the DNA strand breaks induced by NaAsO₂ three hours after treatment in cells from thymus and bone marrow. CONCLUSIONCurcumin can prevent the DNA strand breaks induced by NaAsO₂ in cells from spleen, thymus and bone marrow in vivo.