香虎清妇炎颗粒对大鼠子宫内膜异位症ER、PR、VEGF的影响

目的 研究香虎清妇炎颗粒对实验性子宫内膜异位症大鼠ER、PR、VEGF的影响.方法 用大鼠子宫组织块自体腹壁移植的方法复制EMT模型.模型复制成功后,随机分为香虎清妇炎颗粒高、中、低剂量组(分别给予香虎清妇炎颗粒1.125 g·kg-1、0.75 g·kg-1及0.375 g·kg-1),阳性对照组(给予达那唑0.2 g·kg-1)及模型组;另取大鼠,切除同侧子宫组织,作为假手术对照组.各组大鼠每天用药1次,连续4周.用免疫组化方法检测香虎清妇炎颗粒对异位子宫内膜雌激素受体(ER)、孕激素受体(PR)、血管内皮生长因子(VEGF)表达的影响.结果 与模型组大鼠比较,香虎清妇炎颗粒各个剂量组的异位子宫内膜ER、PR(P<0.05)和VEGF(P<0.01)的表达明显降低.结论 香虎清妇炎颗粒可通过降低异位内膜中ER、PR、VEGF的表达水平,使异位内膜对E2、P的反应性下降,抑制异位内膜血管形成,从而抑制异位内膜的生长.

Influence of XHQFY particles for VEGF,ER and PR on rats with endometriosis

Objective To study the influence of XHQFY particles for vascular endothelial growth factor(VEGF),estrogen receptor (ER) and progesterone receptor (PR) on rats with endometriosis.Methods Establish EMT model by using the rat uterus tissue pieces of abdominal wall autograft.After the success of the model replication,were randomly divided into XHQFY particles high,medium and low dose group(1.125 g·kg-1、0.75 g·kg-1、0.375 g·kg-1),positive control group (danazol 0.2 g·kg-1) and model group.The other rats which were removed of uterus tissue on the same side as the sham control group.Each group was given medicine once a day for 4 weeks.By using immunohistochemical method to detect the expression of ectopic endometrial estrogen receptor (ER),progesterone receptor (PR) and vascular endothelial growth factor (VEGF).Results Compared with model group,the expression of ER,PR (P<0.05) and VEGF (P<0.01)in each dose group of XHQFY particles were significantly decreased.Conclusion XHQFY particles can be reduce the expression of ER,PR and VEGF in ectopic endometrium,make the ectopic endometrium of E2 and P reactivity decreased,thus inhibiting the growth of ectopic endometrium.