IL-17对大鼠心肌梗死后心律失常和心室重塑的影响分析

目的 探讨白细胞介素-17(IL-17)对大鼠心肌梗死后心律失常和心室重塑的影响.方法 液氮冷冻法获取心肌梗死大鼠共72只,按照随机原则分为心梗对照组、IgG1处理组和IL-17抗体处理组,剩余24只大鼠作为空白对照组,四组大鼠组内按照随机原则分别实验处理1个月和2个月,检测四组大鼠心肌IL-17表达水平,记录心律失常和心室重塑相关指标.结果 实验处理后心梗对照组和IgG1处理组大鼠各项指标均未表现出明显差异(P>0.05),心肌梗死各组大鼠心肌IL-17表达水平、心律失常诱发率、Ⅰ/Ⅲ胶原比例均明显高于空白对照组(P<0.05),超声心动图显示心脏功能明显次于空白对照组(P<0.05),IL-17抗体处理组上述指标较心梗对照组和IgG1处理组明显改善(P<0.05),心肌IL-17表达水平与心律失常诱发率呈明显的正相关关系(r=0.89,P<0.05).结论 心肌梗死大鼠心肌IL-17大量表达,对心律失常和心室重塑的形成具有很大的诱导作用.

Analysis the effect of IL-17 expression on arrhythmias and ventricular remodeling in post-myocardial infarction rats

Objective To analyze the effect of IL-17 expression on arrhythmias and ventricular remodeling in post-myocardial infarction rats.Methods Liquid nitrogen cryoinjury method was used to establish 72 myocardial infarction rats.The rats were randomly divided into MI group,IgG1 group and IL-17 antibody group.24 rats were selected as control group.Each group was randomly divided into 1 month and 2 month groups.They were randomly divided into oral medicine group,percutaneous drug group and vaginal drug group.The expression of IL-17 and the indexes in regard to arrhythmias and ventricular remodeling were compared in four groups.Results The indexes showed no obvious difference between MI group and IgG1 group (P>0.05).The expression of IL-17,evoked rate of arrhythmias and Ⅰ/Ⅲ collagen ratio in MI group,IgG1 group and IL-17 antibody group were significantly higher than in control group and there were statistical significantly differences (P<0.05),and the parameters of echocardiogram showed obviously worse result (P<0.05).The above indexes in IL-17 antibody group were improved compared to MI group and IgG1 group (P<0.05).Statistical analysis showed that there was a positive linear correlation between expression of IL-17 and evoked rate of arrhythmias (r=0.89,P<0.05).Conclusion IL-17 expression increases significantly in post-myocardial infarction rats,and may induce the formation of arrhythmias and ventricular remodeling.