HP LC-ELSD法测定灵芝中灵芝酸A的含量

目的 建立测定灵芝药材中灵芝酸A质量分数的HPLC-ELSD法.方法 采用Welch XtimateTM-C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-体积分数0.01%醋酸(体积比37:63),流速为1 mL/min,柱温为30℃,进样量为10μL;蒸发光散射器漂移管温度为105℃,空气流速为2.8 L/min.结果 灵芝酸A的进样量在0.8928~5.3568μg范围内,峰面积的对数值与进样量的对数值具有良好的线性关系;平均加样回收率为95.81%;不同批次的灵芝样品中灵芝酸A的质量分数范围为0.2143~2.8942 mg/g,结果差异较大.结论 本法操作简单、结果准确、重复性好,为灵芝的质量控制提供参考方法.

Determination of ganoderic acid A in Ganoderma lucidum by HPLC-ELSD

Objective To establish an HPLC-ELSD method for determination of ganoderic acid A in Ganoderma lucidum. Methods The separation was performed on a Welch XtimateTM-C18 column ( 4.6 mm× 250 mm, 5 μm) , with the mobile phase of acetonitrile-0.01% acetic acid solution( 13:87) at a flow rate of 1 mL/min. The column temperature was 30℃ and the injection volume was 10μL. ELSD, the temperature of the drift tube was set at 105 ℃, and the flow rate of nebulizing gas was 2. 8 L/min. Results The log values of peak areas had a good linear relationship with the numerical value of the injection quantity, the linear response range was 0. 8928~5. 3568 μg for ganoderic acid A and the average recoveries was 95.81%. The content range of ganoderic acid A was 0.2143~2.8942 mg/g for Ganoderma lucidum from different batches. Conclusion The method is simple, accurate and credible, which can provide a reference method for the quality control of Ganoderma lucidum.