| 鞘氨醇激酶2(SphK2)基因敲除小鼠繁殖及基因型鉴定 |
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| 引用本文： | 李柠,李昌正,杨桂智,颜思珊,李锐,刘冰,兰天. 鞘氨醇激酶2(SphK2)基因敲除小鼠繁殖及基因型鉴定[J]. 广东药学院学报, 2017, 33(4). DOI: 10.16809/j.cnki.2096-3653.2017050901 |
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| 作者姓名： | 李柠 李昌正 杨桂智 颜思珊 李锐 刘冰 兰天 |
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| 作者单位： | 1. 广东药科大学 药学院,广东 广州,510006;2. 广东药科大学 基础学院,广东 广州,510006;3. 广东药科大学 药学院,广东 广州510006;广东药科大学 基础学院,广东 广州510006 |
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| 基金项目： | 广东省自然科学基金重点项目，广东省科技计划项目 |
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| 摘 要： | 目的 繁殖和快速鉴定鞘氨醇激酶2(SphK2)基因敲除小鼠.方法 将SphK2敲除杂合子小鼠进行配种繁殖,提取子代小鼠尾部DNA,用PCR方法检测子代小鼠基因型.结果 SphK2杂合子小鼠的饲养及繁殖均获得成功,获得子代小鼠,基因型分别为杂合子(SphK2+/-)、纯合子(SphK2-/-)和野生型(SphK2+/+).结论 SphK2基因敲除小鼠的繁育和鉴定均获得成功,为进一步研究SphK2生物学功能提供了理想的动物模型.
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| 关 键 词： | SphK2 基因敲除小鼠 基因型 |
Breeding and genotyping of Shingosine Kinase 2 knockout mice |
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| LI Ning,LI Changzheng,YANG Guizhi,YAN Sishan,LI Rui,LIU Bing,LAN Tian. Breeding and genotyping of Shingosine Kinase 2 knockout mice[J]. Academic Journal of Guangdong College of Pharmacy, 2017, 33(4). DOI: 10.16809/j.cnki.2096-3653.2017050901 |
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| Authors: | LI Ning LI Changzheng YANG Guizhi YAN Sishan LI Rui LIU Bing LAN Tian |
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| Abstract: | Objective To breed and identify SphK2 gene knock-out mice. Methods SphK2 knockout heterozygous mice were bred and DNA of the offspring mice was extracted. The genotypes of the offspring mice were analyzed by PCR. Results The feeding and breeding of SphK2 heterozygous mice were successful, and the offspring mice were obtained. The genotypes included heterozygous ( SphK2+/-) , homozygous ( SphK2-/-) and wild type ( SphK2+/+) . Conclusion The methods of breeding and genotyping of SphK2 knockout mice have been successful established, which provides an ideal animal model for further study of the biological function of SphK2. |
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| Keywords: | SphK2 gene knockout mice genotype |
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