藿朴夏苓汤治疗糖尿病肝病大鼠的实验研究

目的 研究藿朴夏苓汤对链脲佐菌素(STZ)+CCl4合并诱导的糖尿病肝病的治疗作用.方法 采用腹腔注射STZ+灌胃40%的CCl4法建立糖尿病肝病大鼠模型,将大鼠随机分为正常组、模型组、藿朴夏苓汤组和格列本脲组.给药6周后,测定血糖、谷丙转氨酶(ALT)、谷草转氨酶(AST)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)等指标,研究藿朴夏苓汤对糖尿病肝病大鼠的血糖、肝功能、血脂及肝组织病理变化等的影响.结果 与模型组大鼠相比,藿朴夏苓汤能显著降低大鼠的空腹血糖(BG)(P<0.01),ALT、AST、TG、TC以及LDL的含量显著降低(P<0.01),HDL含量显著增高(P<0.01),进而抑制大鼠肝内Ⅰ型胶原蛋白的形成,导致改善大鼠肝组织病理.结论 藿朴夏苓汤具有明显改善STZ+CCl4诱导的糖尿病肝病大鼠的肝损伤作用.

Protective effect of Huo-pu-xia-ling-tang in diabetic hepatopathy rats

Objective To study the therapeutic effect of Huo-pu-xia-ling-tang ( HPXLT) on liver injury of diabetic hepatopathy ( DH) rats induced by streptozocin ( STZ) and CCl4. Methods Rats were treated i.p. with STZ and administered intragastrically CCl4 for 6 weeks to induce DH rat model. Rats were randomly divided into the normal group, the model group, HPXLT and glibenclamide groups. After treatment with HPXLT for 6 weeks, the levels of blood glucose ( BG) , serum alanine aminotransferase ( ALT) , aspartate aminotransferase ( AST) , triglyceride ( TG) , cholesterol ( TC) , high density lipoprotein cholesterol ( HDL-C) and low density lipoprotein cholesterol ( LDL-C ) were measured. The tissue pathological changes and collagenase I expression were also assessed by HE staining and immunohistochemistry, respectively. Results Compared with the model group, HPXLT treatment reduced the levels of BG( P<0.01) , ALT, AST, TG, TC and LDL-C levels ( P<0. 01 ) , but increased the level of HDL-C. Besides, HPXLT also inhibited the production of collagen I and relieved the hepatic pathological damages. Conclusion HPXLT exhibits a protective effect on liver injury in DH rats.