HPLC法测定八味肉桂胶囊4种成分的含量

目的 建立同时测定八味肉桂胶囊中芍药苷、肉桂酸、胡椒碱、去氢木香内酯含量的方法.方法 采用5C18-MS-Ⅱ(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-0.1%乙酸梯度洗脱;流速为1.0 mL/min;检测波长为230 nm;柱温为35℃.结果 八味肉桂胶囊中芍药苷、肉桂酸、胡椒碱、去氢木香内酯能完全分离,质量浓度与其峰面积之间均呈良好的线性关系,相关系数均大于0.999.芍药苷平均回收率为98.26%,RSD为1.91%(n=6);肉桂酸平均回收率为100.54%,RSD为2.14%(n=6);胡椒碱平均回收率为100.46%,RSD为2.41%(n=6);去氢木香内酯平均回收率为98.42%,RSD为2.48%(n=6).结论 该法简便、准确,专属性强,可用于八味肉桂胶囊的质量控制.

Determination of effective constituents in Bawei Rougui capsules by HPLC

Objective To establish a simultaneous determination method for four effective materials in Bawei Rougui capsules. Methods The chromatographic method was carried out on a 5C18-MS-Ⅱ( 4. 6 mm × 250 mm, 5 μm) column at temperature of 35 ℃ and the mobile phase was consisted of acetonitrile and 0.1%acetic acid with the flow rate of 1.0 mL/min. The UV detection wavelength was at 230 nm. Results In the quantitative analysis, four compounds were simultaneously separated completely. All the calibration curves and the regression equations showed good linear correlation between concentration and peak area with the correlation coefficients more than 0. 999. The average recovery results with relative standard deviation ( RSD) were listed as follows: paeoniflorin 98.26%( RSD=1.91%, n=6) , cinnamic acid 100.54%( RSD=2.14%, n=6) , piperine 100.46% ( RSD=2.41%, n=6) , dehydrocostus latone 98.42% ( RSD=2.48%, n=6) . Conclusion The HPLC method was validated to be specific, linear, precise and accurate, which could be used to for the quality control of Bawei Rougui capsules.