芭蕉花活性成分提取及其体外生物活性研究

目的 研究芭蕉花的活性成分及其体外生物活性.方法 通过硅胶柱色谱、大孔树脂、TLC分离等对芭蕉花提取物进行分离,并用高效液相色谱,结合质谱分析等方法,进行成分鉴定;采用体外MTT法进行芭蕉花活性组分对抑制肝癌BEL-7402细胞增殖作用的筛选,对抑制AngⅡ诱导的A7r5平滑肌细胞增殖作用进行活性筛选.结果 通过液-质分析,从芭蕉花乙酸乙酯提取物和石油醚提取物中发现其中有一种化合物可能为豆甾醇.MTT筛选发现芭蕉花石油醚提取物的4种萃取物组分中,乙酸乙酯相和石油醚相对肝癌BEL-7402细胞具有显著的增殖抑制作用(P<0.01),且呈一定的剂量和时间依赖性;芭蕉花的3种提取物组分中,乙酸乙酯相和石油醚相对AngⅡ诱导的A7r5平滑肌细胞增殖具有显著的抑制作用(P<0.01),且其抑制作用呈一定的剂量和时间依赖性.结论 芭蕉花石油醚提取物的石油醚萃取部位和乙酸乙酯萃取部位对肝癌BEL-7402细胞具有显著的增殖抑制作用,72 h的IC50分别为67.4μg/mL和34.5μg/mL,且石油醚提取物和乙酸乙酯提取物对AngⅡ诱导的A7r5平滑肌细胞增殖有抑制作用,72 h的IC50为55.5μg/mL.从芭蕉花提取物中分离出单体豆甾醇,为芭蕉花活性成分的深入研究提供了参考.

Analysis of the chemical compositions of the flower of Musa basjoo Sieb. et Zucc and their bioactivity in vitro

Objective To investigate the chemical constituents and biological activities of the flower of Musa basjoo Sieb. et Zucc. Methods The chemical compositions of flower of Musa basjoo Sieb. et Zucc were isolated by silica gel column chromatography, macroporous resin and TLC, purified by HPLC, and identified by MS technology. MTT assay was used to test the effect of extracts of Musa basjoo Sieb. et Zucc on BEL-7402 cell proliferation and Ang Ⅱ-induced A7r5 cell proliferation. Results One of the chemical constituents, stigmasterol, was identified by LC-MS from ethyl acetate and petroleum ether phase constituents. The petroleum ether phase and ethyl acetate phase from the petroleum ether extract significantly inhibited the proliferation of BEL-7402 cells in a dose-dependent manner ( P<0. 01 ) . The petroleum ether extract and ethyl acetate extract reduced the proliferation of A7r5 cells induced by AngⅡin a dose-and time-dependent manner ( P<0.01) . Conclusion The petroleum ether phase and ethyl acetate phase from the petroleum ether extract inhibit the proliferation of BEL-7402 cells. The petroleum ether extract and ethyl acetate extract inhibit the proliferation of A7r5 cells induced by AngⅡ. Stigmasterol has been isolated from Musa basjoo Sieb. et Zucc, which provides a reference for further study of its biological activity.