药物拮抗单核细胞粘附及迁移于肿瘤坏死因子处理的脑血管内皮细胞单层

研究肿瘤坏死因子(TNF)影响单核细胞与体外培养的牛脑微血管内皮细胞(BCMEC)粘附，迁移的动力学过程及药物的保护作用. 利用培养在胶原层上的单层内皮细胞，通过对粘附于内皮细胞单层上的单核细胞及胶原层中的单核细胞计数，测定单核细胞粘附及迁移. TNF(100 kU·L^-1)可促进单核细胞与BCMEC的粘附及迁移，其促粘附与迁移作用具明显的时效关系. TNF促粘附达最大效应时间为2 h，较对照提高120.7%；TNF促迁移作用1 h达到坪值，提高91.9%. 药物戊烯氧呋豆素(imperatorin, IMP), 己酮可可碱(pentoxifylline, PTX)抑制由TNF引起的单核细胞与BCMEC的粘附及迁移，在浓度为1, 10及100 μmol·L^-1时，其粘]附抑制率分别为73.1%, 87.7%, 93.3%和73.6%, 87.2%, 89.0%；迁移抑制率分别为17.4%, 43.4%, 68.1%和37.7%, 49.3%, 62.3%. IMP和PTX能抑制单核细胞与TNF诱导的BCMEC的粘附及迁移.

Antagonistic effects of drugs on monocyte adhesion to and migration through tumor necrosis factor treated cerebromicrovascula endothelial cell monolayer in vitro

Antagonistic effects of pentoxifylline(PTX) and imperatorin(IMP) on adhesion of monocytes to and migration through tumor necrosis factor (TNF)-stimulated bovine cerebromicrovas cular endothelial cell(BCMEC) layer in vitro were studied. The adhesion and migration of bovine monocytes were measured using a three-dimensional model system comprising BCMEC cultured on collagen gels in vitro. The results showed that prestimulation of BCMEC with TNF(100 kU·L^-1) enhanced monocytes adhesion to and migration through TNF- stimulated BCMEC layers. Both adhesion and migration of monocytes were increased with time. After 2 h or 1 h incubation of BCMEC with TNF, adhesion or migration reached maximum (120.7% and 91.9% respectively). At concentrations from 1 to 100 μmol·L^-1, both IMP and PTX were found to inhibit the monocyte adhesion and migration. Their maximal adhesion inhibitory effects were 93.3% and 89.0% at 100 μmol·L^-1, respectively, and their maximal migration inhibitory effects were 68.1% and 62.3% at 100 μmol·L^-1, respectively.