应用"双质控法"、加强"即刻法"室内质控

目的应用“双质控法”,加强、完善“即刻法”室内质控,并用于抗-HIV抗体检测的质量监控.方法采用ELISA法进行HIV抗体检测.每次更换试剂时,使用原试剂和新批号试剂同板检测3次,并采用同一质控血清.原试剂的质控结果参与原数据组按“即刻法”分析,新试剂的质控结果参与原试剂数据组按“即刻法”分析,3次均不向负值偏移至告警或失控时,从第3次开始新数据组用“即刻法”分析.更换质控血清时,两种质控血清同板检测3次,即在同一试剂板中分别加入原质控血清和新质控血清;原质控血清的检测结果参与原数据组按“即刻法”分析,新质控血清的检测结果从第3次开始新数据组用“即刻法”分析.结果采用“双质控法”较好地解决了“即刻法”开始几次检测结果无法控制的状况.结论 “双质控法”进一步加强、完善了“即刻法”在ELISA检测中的质量监控作用.

Strengthening lab quality control with instant method by double quality control

Objective To approach the way of strengthening lab quality control with instant method by double quality control and to apply it in the anti-HIV antibody test.Methods Anti-HIV antibody was examined by ELISA method.The same plate was tested three times with original agent and new agent,using the same quality control serum,while making change of agent batch number.The quality control result of original agent was put into the original data group and then analyzed with instant method.The same plate was tested three times with original quality control serum and new quality control serum while making change of quality control serum.The result of test with original quality control serum was put into the original data group and analyzed with instant method.The result of test with new quality control serum was analyzed with the instant method from the third new data group.Results The double quality control method solved the problem of uncontrolled test result in the initial times with the instant method.Conclusion The double quality control method further strengthens the quality control with the instant method in ELISA test.