| 联合应用激光微切和实时定量RT-PCR检测单个胰岛的基因表达 |
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| 引用本文： | 石欣,高乃荣,霍明东,胡浩霖,Friess Helmut,Buchler Markus. 联合应用激光微切和实时定量RT-PCR检测单个胰岛的基因表达[J]. 中华内分泌代谢杂志, 2003, 19(3): 242-243,U007 |
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| 作者姓名： | 石欣 高乃荣 霍明东 胡浩霖 Friess Helmut Buchler Markus |
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| 作者单位： | 1. 210009,南京,东南大学附属中大医院普外科
2. 德国海德堡大学医院普外科 |
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| 摘 要： | 在显微镜下用紫外激光微切机将单个胰岛从胰腺切片中切下，提取RNA后逆转录成cDNA并在实时定量RY—PCR中得到有效的扩增，其表达量与细胞数量成正比。
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| 关 键 词： | 胰岛 胰腺切片 激光微切 实时定量 RT—PCR 检测 基因表达 |
Gene expression analysis in a single islet: real-time quantitative RT-PCR after laser microdissection |
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| Friess Helmut,Buchler Markus. Gene expression analysis in a single islet: real-time quantitative RT-PCR after laser microdissection[J]. Chinese Journal of Endocrinology and Metabolism, 2003, 19(3): 242-243,U007 |
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| Authors: | Friess Helmut Buchler Markus |
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| Abstract: | Single islet was microdissected from pancreatic tissue section under microscope by ultraviolet laser micromanipulator. RNA extracted from above islet was effectively amplified by real time quantitative RT-PCR. The quantitation of expression was compatible with the number of cells. |
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| Keywords: | Islet of Langerhans Gene expression Laser microdissection Real-time quantitative polymerase chain reaction |
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