彩色乳胶免疫层析法检测单纯疱疹病毒1,2型抗原临床应用分析

目的探讨彩色乳胶免疫层析法检测单纯疱疹病毒(HSV)1和2型抗原的临床应用价值。方法采用彩色乳胶颗粒免疫层析法检测各种水疱、溃疡和其他皮损中的HSV-1和2型抗原,同时用实时荧光定量PCR法进行对照。结果免疫层析法可检出≥1×106DNA拷贝/mL的HSV-1型混悬液和≥5×105DNA拷贝/mL的HSV-2型混悬液。132例标本中,免疫层析法检出HSV阳性43例,PCR检出HSV阳性50例。以PCR法为金标准,免疫层析法的灵敏度、特异度、阳性预测值和阴性预测值分别为84.00%,98.78%,97.67%和91.01%。结论免疫层析法检测HSV敏感性和特异性高,具有方便、快速和经济的特点,适于有症状患者的及时检测,对疱疹感染早期诊断和及时治疗有重要作用。

Analysis of Clinical Application of Immuno-chromatographic Assay for Detection of HSV

Objective To approach the clinical value of dyed latex beads immuno-chromatographic assay (ICA)for detection of Herpes simplex virus(HSV) 1 and 2 antigens. Methods The dyed latex beads ICA were used to detect HSV 1 and 2 antigens in blister, ulcer and other cutaneous lesions.The detection of HSV by PCR was taken as a control. Results ICA may detect more than and equal 1×106DNA copy/mL of HSV-1 mixture, and more than and equal 5×105DNA copy/mL of HSV-2 mixture. In detected 132 cases, 43 cases were positive by ICA, and 50 cases were positive by PCR. When PCR was taken as the golden standard, the sensitivity, specificity, positive predictive value and negative predictive value were 84.00%, 98.78%, 97.67% and 91.01% respectively. Conclusion The sensitivity and specificity of ICA to detect HSV is high. ICA is convenient, rapid and economical. ICA may detect HSV promptly for symptomatic patients and play an important role in early diagnosis and timely treatment for HSV infection.