超临界二氧化碳萃取气相色谱——质谱联用分析东当归挥发油化学成分

目的对吉林省延边地区产东当归的挥发油化学成分进行分析。方法采用超临界CO2萃取技术对东当归中挥发油成分进行提取,用GC-MS联用技术分离鉴定其化学组成,计算其相对百分含量。结果超临界CO2萃取物为黄棕色油状物,得率为0.9%,用GC-MS联用技术从中鉴定出亚油酸乙酯、5,5-二甲基-双环(6,3,0)-1,7-十一烯-3-酮等30种成分,占挥发油总含量65.66%;萃取物经硅胶柱色谱分离纯化,从中鉴定出十八酸乙酯等11种成分,占总含量的85.1%。结论首次从东当归挥发油当中分离鉴定出5,5-二甲基-双环(6,3,0)-1,7-十一二烯-3-酮等10种化学成分,超临界CO2萃取与传统方法提取的东当归中挥发油化学成分有显著不同。

Extraction and Analysis of Constituents of Essential Oil from Angelica acutiloba Kitag with Supercritical Fluid CO2 and GC -MS Method

Objective To study the chemical components of the essential oil from the Angelica acutiloba Kitag of Yanbian region in Jilin.Methods The essential oil of the Angelica acutiloba Kitag was extracted by SFE-CO2 and by the silica gel column separately,the GC-MS method was used for the analysis of extracts.Results The SFE-CO2 extracted the yellow brown oil,the rate was 0.9%.30 components were identified which represented 65.66% of the total essential oil includding 9.12-Octadecadienoic acid,ethyl ester and ect.11 components were identified which represented 85.1% of the total essential oil by the silica gel column separately.Conclusion 10 components were identified which included Bicyclo(6,3,0) undeca-dien-3-one,5,5-dimethyl ect.of the essential oil from Angelica acutiloba Kitag were analyzed for the first time.The SFE-CO2 extracted the volatile oil chemistry composition in the Angelica acutiloba Kitag that withdraws with traditional method have already shown the marked difference.