维生素E对微波辐照后视网膜神经节细胞形态的影响

为探讨微波对体外培养的猪视网膜神经节细胞形态的影响及维生素 E(VE)的防护作用 ,为微波致视网膜损伤的研究提供一定的实验依据。用体外培养猪视网膜神经节细胞方法 ,光镜及电镜观察其形态结构 ,加入不同浓度 VE,用微波理疗机于微波屏蔽室内以 30 m W/ cm2 功率密度辐照 1h,辐照后立即于光镜下观察其形态变化 ,电镜观察其超微结构 ,比较辐照前后及加入 VE(10、2 0和 40 g/ L)后视网膜神经节细胞的变化。结果显示微波辐照后 ,细胞有聚集现象 ,部分细胞轴突消失 ,电镜可见线粒体及内质网肿胀 ,VE各组光镜下细胞形态变化不明显 ,电镜显示线粒体轻度肿胀 ,嵴完整。结果表明微波可引起视网膜神经节细胞形态损伤 ,VE可在一定程度上减轻微波对视网膜神经节细胞的损伤

Effect of vitamin E on morphological variation of retinal ganglion cells after microwave radiation

To determine the morphological variation in the primary cultured pig retinal ganglion cells induced by microwave and the protection of VE can supply some experiment foundation for study of effect of microwave and its protection. Retinal ganglion cells of pig were cultured in vitro and added VE of different concentration, Each group was taken after 30mW/cm 2 microwave intensity radiated for 1h in shielded room by 2450MHz continuous wave physiotherapy machine. Immediately after radiation, the morphological variation of cells was observed by optics microscope and transmission electronic microscope.The result showed that a portion of cells congregated, with their axon disappeared after radiation. Mitochondria and endoplasmic reticulum are detected swelling by transmission electronic microscope. The results showed that A poptosis cells can be observed. Cells of VE added groups had not obvious changes with optics microscope, but could be found that mitochondria swelling lightly and integrate mitochondria cristae by transmission electronic microscope.The results showed that microwave induced the morphological damage in primary cultured retinal ganglion cells, VE could reduced the damage of retina ganglion cells by microwave in some extent.