Identification of human blood in mosquitoes (Diptera: Culicidae) using nonradioactive DNA dot blot hybridization. |
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Authors: | C Sato Y Furuya M Harada S Suguri |
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Affiliation: | Department of Biology, Kagawa Medical School, Japan. |
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Abstract: | A dot blot hybridization procedure was developed to detect human blood meals in engorged mosquitoes. A biotinylated DNA probe allowed the detection of 10-100 ng of human DNA, discriminated well between human and nonhuman sources of blood, and cross-reacted only with monkey DNA. Results showed that this method was a specific and sensitive technique for the identification of blood meals up to 100 h after ingestion. The nonisotopic label offers easy handling without the problems inherent in the use of radioisotopes, and it can be adapted for use in routine field tests. |
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