Early Morphological Changes in the Thalamocortical Projection Onto the Parietal Cortex Following Ablation of the Motor Cortex in the Cat

Following a previous report that the cerebellar-induced cerebral response in the parietal cortex changes acutely after ablation of the frontal motor cortex, the present experiments tested whether morphological changes of the thalamo-parietal projection occur after ablation of the motor cortex. Anterograde and retrograde tracing with wheat germ agglutinin conjugated with horseradish peroxidase was used in intact and lesioned cats. The thalamocortical projection was labeled anterogradely by tracer injection into the thalamic ventral anterior and ventral lateral (VA-VL) nuclear complex that mainly relays the cerebello-cerebral projection, and thalamic neurons were labeled retrogradely by injection of the tracer into the parietal cortex. The labeled terminals in the parietal cortex of the intact animals were distributed densely in layer I and sparsely in layers III–IV, whereas those of the lesioned animals were distributed densely in layers I and III–IV. The distribution of the retrogradely labeled neurons after multiple tracer injections in layers III–IV of the parietal cortex was different in the intact and lesioned cats. In the intact animals, the labeled neurons were distributed sparsely in the central lateral nucleus and in the lateral posterior and pulvinar nuclear complex. In contrast, after ablation of the frontal cortex, the labeled neurons were also observed in the VA-VL nuclear complex. These differences between the intact and lesioned animals were detectable within 48 h after the lesion.