| 煤焦沥青烟提取物致人支气管上皮细胞BEAS-2B染色体不稳定性研究 |
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| 引用本文： | 李智涛,冯艳铭,王威,王丽霞,赵勇,祝寒松,吴卫东,吴逸明. 煤焦沥青烟提取物致人支气管上皮细胞BEAS-2B染色体不稳定性研究[J]. 工业卫生与职业病, 2011, 0(3): 129-136 |
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| 作者姓名： | 李智涛 冯艳铭 王威 王丽霞 赵勇 祝寒松 吴卫东 吴逸明 |
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| 作者单位： | 郑州大学公共卫生学院;河南科技大学医学院; |
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| 基金项目： | 国家自然科学基金(30872095) |
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| 摘 要： | 目的 建立煤焦沥青烟提取物诱导的人支气管上皮细胞BEAS-2B株的恶化模型;观察不同时期细胞的染色体不稳定性与细胞恶性转化之间的关系.方法用四唑蓝(MTT)法测定煤焦沥青烟提取物的细胞毒性,以煤焦沥青烟提取物诱导并观察BEAS-2B细胞传代转化过程中的形态学改变;软琼脂克隆形成实验检测细胞恶性转化能力,流式细胞术检测细...
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| 关 键 词： | 煤焦沥青烟提取物 细胞恶性转化 染色体不稳定 |
Chromosomal Instability of Human Tracheal Epithelial Cells BEAS-2B Induced by Extract of Coal Tar Pitch Fume |
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| LI Zhi-tao,FENG Yan-ming,WANG Wei,WANG Li-xia,ZHAO Yong,ZHU Han-song,WU Wei-dong,WU Yi-ming. Chromosomal Instability of Human Tracheal Epithelial Cells BEAS-2B Induced by Extract of Coal Tar Pitch Fume[J]. Industrial Health and Occupational Diseases, 2011, 0(3): 129-136 |
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| Authors: | LI Zhi-tao FENG Yan-ming WANG Wei WANG Li-xia ZHAO Yong ZHU Han-song WU Wei-dong WU Yi-ming |
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| Affiliation: | LI Zhi-tao,FENG Yan-ming,WANG Wei,WANG Li-xia,ZHAO Yong,ZHU Han-song,WU Wei-dong,WU Yi-ming( College of Public Health,Zhengzhou University,Zhengzhou 450001 China) |
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| Abstract: | Objective To establish a cell model of malignant transformation with human bronchial epithelial cells BEAS-2B induced by extract of coal tar pitch fume and to observe the relationship between chromosome instability and the cell malignant transformation in different cell generations.Methods MTT assay was used to determine the cytotoxicity of the extract of coal tar pitch fume.After induced by coal tar pitch fume extract at 2.0 mg/L,the morphological changes of BEAS-2B cells were observed in their passage and... |
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| Keywords: | Coal tar pitch fume extract Malignant transformation Chromosomal instability |
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