| Evaluation of a sulfonated DNA probe (sulfoprobe) for diagnosis of falciparum malaria |
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| 引用本文： | 缪为民,管惟滨,徐晓春,周元昌,陆德如,董蓓华,陈蕊雯. Evaluation of a sulfonated DNA probe (sulfoprobe) for diagnosis of falciparum malaria[J]. 中国人民解放军军医大学学报, 1993, 0(3) |
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| 作者姓名： | 缪为民 管惟滨 徐晓春 周元昌 陆德如 董蓓华 陈蕊雯 |
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| 作者单位： | Departments of Parasitology Second Military Medical University Shanghai，200433，Departments of Parasitology Second Military Medical University，Shanghai，200433，Departments of Parasitology Second Military Medical University，Shanghai，200433，Departments of Parasitology Second Military Medical University，Shanghai，200433，Departments of Molecular Genetics Second Military Medical University，Shanghai，200433，Departments of Immunology，Second Military Medical University，Shanghai，200433 |
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| 摘 要： | In this study,the DNA probe pPF14 was nonradioactively labelled by sulfo-modifica-tion,and used in a dot blot hybridization assay for detection of P.falciparum.The resultsshowed that the sulfoprobe successfully detected 25pg purified DNA and 0.001% parasitemia ofcultured P.falciparum,and did not react with human leukocyte DNA.In the study of 179clinical blood samples of malaria patients from Yunnan province,the DNA probe results corre-lated well with blood smear ones.Of 107 P.falciparum samples determined by microscope ex-amination,99 were positive by sulfoprobe (92.5%);Of 72 P.vivax samples,1 was crosslyreacted;no cross reactions were found with any of 48 normal blood samples.It is indicated thatsulfoprobe may be useful in specific diagnosis and epidemiological investigation of P.falciparuminfection.
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Evaluation of a sulfonated DNA probe (sulfoprobe) for diagnosis of falciparum malaria |
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| Miao Weiming Guan Weibin Xu Xiaochun Zhou Yuanchang Lu Deru Dong Peikua Cken Ruiwen Departments of Parasitology,Molecular Genetics,and Immunolog,Second Military Medical University,Shanghai.. Evaluation of a sulfonated DNA probe (sulfoprobe) for diagnosis of falciparum malaria[J]. Journal of Medical Colleges of PLA(China), 1993, 0(3) |
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| Authors: | Miao Weiming Guan Weibin Xu Xiaochun Zhou Yuanchang Lu Deru Dong Peikua Cken Ruiwen Departments of Parasitology Molecular Genetics and Immunolog Second Military Medical University Shanghai. |
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| Affiliation: | Miao Weiming Guan Weibin Xu Xiaochun Zhou Yuanchang Lu Deru Dong Peikua Cken Ruiwen Departments of Parasitology,Molecular Genetics,and Immunolog,Second Military Medical University,Shanghai.200433 |
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| Abstract: | In this study,the DNA probe pPF14 was nonradioactively labelled by sulfo-modifica- tion,and used in a dot blot hybridization assay for detection of P.falciparum.The results showed that the sulfoprobe successfully detected 25pg purified DNA and 0.001% parasitemia of cultured P.falciparum,and did not react with human leukocyte DNA.In the study of 179 clinical blood samples of malaria patients from Yunnan province,the DNA probe results corre- lated well with blood smear ones.Of 107 P.falciparum samples determined by microscope ex- amination,99 were positive by sulfoprobe (92.5%);Of 72 P.vivax samples,1 was crossly reacted;no cross reactions were found with any of 48 normal blood samples.It is indicated that sulfoprobe may be useful in specific diagnosis and epidemiological investigation of P.falciparum infection. |
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| Keywords: | DNA probe non-radioactive labelling sulfomodification P.falciparum malariadiagnosis |
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