| 乙酰肝素酶反义寡核苷酸对胃癌细胞侵袭和转移的影响Ⅱ.胃癌细胞SGC-7901中乙酰肝素酶基因和CD44v6表达的关系 |
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| 引用本文： | 周世庆,汪素文,朱靖宇,张淑红,真岩波,张伯新.乙酰肝素酶反义寡核苷酸对胃癌细胞侵袭和转移的影响Ⅱ.胃癌细胞SGC-7901中乙酰肝素酶基因和CD44v6表达的关系[J].山东大学学报(医学版),2005,43(9). |
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| 作者姓名： | 周世庆 汪素文 朱靖宇 张淑红 真岩波 张伯新 |
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| 摘 要： | 目的:探讨乙酰肝素酶(HPA)反义寡核苷酸(AS-ODN)对CD44v6表达的影响.方法:采用脂质体介导HPA AS-ODN转染人胃癌细胞株SGC-7901,RT-PCR检测HPA mRNA的表达,采用免疫细胞化学SABC法观察细胞内CD44v6表达的变化.结果:脂质体介导转染HPA AS-ODN 48 h后,SGC-7901胃癌细胞内HPA mRNA表达下降,CD44v6阳性表达率转染前69.4%,转染不同浓度的AS-ODN后分别为56.5%,54.1%,46.5%和41.3%.转染后细胞CD44v6表达明显减少(P＜0.01).结论:HPA AS-ODN可以有效抑制胃癌细胞CD44v6的表达.
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| 关 键 词： | 乙酰肝素酶 蛋白 CD44v6 寡核苷酸类 反义 胃肿瘤 |
Influence of invasion and metastasis in human gastric carcinoma cell by heparanase antisens oligodeoxynucleotide Ⅱ. Relationship between the CD44v6 expression and heparanase gene in gastric carcinoma cell line SGC-7901 |
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| ZHOU Shi-qing,WANG Su-wen,ZHU Jing-yu,ZHANG Shu-hong,ZHEN Yan-bo,ZHANG Bo-xin.Influence of invasion and metastasis in human gastric carcinoma cell by heparanase antisens oligodeoxynucleotide Ⅱ. Relationship between the CD44v6 expression and heparanase gene in gastric carcinoma cell line SGC-7901[J].Journal of Shandong University:Health Sciences,2005,43(9). |
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| Authors: | ZHOU Shi-qing WANG Su-wen ZHU Jing-yu ZHANG Shu-hong ZHEN Yan-bo ZHANG Bo-xin |
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