脑源性神经营养因子对大鼠视网膜节细胞损伤的保护作用

目的：观察脑源性神经营养因子（brain-derived neurotrophic factor,BDNF)对Sprague-Dawly(SD)大鼠视神经损伤后视网膜节细胞（retinal ganglin cells,RGC)存活的作用。方法：将SD大鼠分为正常对照组，夹伤对照组，溶剂对照组，BDNF治疗组4组，每组20只眼。荧光金逆行标记RGC后7天，除正常组外行球后视神经夹伤，将100ng BDNF注入BDNF治疗组大鼠玻璃体腔内，分别于5，7，14，21，28天行RGC计数。结果：视神经夹伤后第7天RGC开始减少，14天时降至正常对照的70．2％，28天时降至40．5％。与正常组相比BDNF治疗组14天时RGC数开始减少，但7、14、21、28天RGC数均明显多于夹伤组（P＜0．01）。结论：在视神经夹伤后眼内注射BDNF能减少RGC的死亡，对RGC损伤有保护作用。

Protective Effects of Brain-derived Neurotrophic Factor on Injured Retinal Ganglion Cells of Rats

Purpose: To investigate in vivo the survival of retinal ganglion cells (RGC) after optic nerve crush with intraocular injection of Brain-derived neurotrophic factor (BDNF). Methods: Sprague-Dawly(SD) rats were divided into normal control, crush control, medium treatment and BDNF treatment group, and every group had 20 eyes. RGC of adult rats were labeled with fluorogold. Seven days later, the optic nerve was intraorbitally crushed and BDNF was injected into the vitreous. Five, 7, 14, 21 and 28 days after crush, the numbers of RGC were counted. Results: The densities of RGC began to decrease 7 days after injury, the number of RGC was 70. 2% and 40. 5% of normal controls on days 14 and 28, respectively. In the group with BDNF injection, RGC densities decreased on days 7, but RGC densities were much higher than that of controls on 7, 14, 21, and 28 days after injury (P < 0. 01). Conclusion: BDNF administered intraocularly at the time of crush can protect RGC from injury and cell death. Eye Science 2000; 16: 231-234.