小型香猪复方壳多糖组织工程皮肤的构建及组织学特点

目的评价制备的小型香猪复方壳多糖组织工程皮肤在组织学方面的特性,为其修复皮肤缺损创面提供实验依据。方法采用传代培养的角质形成细胞和成纤维细胞作为种子细胞,I型胶原作为真皮基质。采用气—液界面方式进行培养,通过苏木精—伊红(HE)染色、比较小型香猪的体外构建组织工程皮肤与正常皮肤的组织学特征,包括表皮、真皮结构。结果制备的复方壳多糖组织工程皮肤表皮细胞增殖活跃,分层分化良好,厚约150μm,真皮层细胞生长正常,排列有序,与机体皮肤结构相似。结论复方壳多糖组织工程皮肤组织结构良好,符合新型皮肤替代物在治疗皮肤缺损时的组织学要求。

Construction and histologic characteristics of swine compound chitosan tissue-engineered skin

Objective To establish a method of constructing swine compound chitosan tissue-engineered skin in vitro and to evaluate the histologic characteristics of this kind artificial skin,and to provide experimental evidence for applying tissue-engineered skin in the repair of full skin loss. Methods The serially passed swine KC and FB were used to fabricate the compound chitosan tissue engineered skin. The histological alterations, including epidermis,dermis and basement membrane of the tissue-engineered skin,were observed,hematoxylin and eosin staining,and compared with the swine skin. Results The keratinocytes formed a fine stratified squamous epithelium,about a thickness of 150 micrometers,with the presence of basal,spinous,granular and corneous cell layers. The fibroblasts in dermis were normal in growth and disposition similar to skin structure in vivo. Conclusion The compound chitosan tissue-engineered skin may be a novel skin equivalent applied in clinic in the future for its perfect histologic characteristics.