| 青藤碱脂质体包封率的测定 |
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| 引用本文： | 周莉玲,王岩,刘清飞,凌家俊.青藤碱脂质体包封率的测定[J].中国中药杂志,2006,31(9):731-734. |
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| 作者姓名： | 周莉玲 王岩 刘清飞 凌家俊 |
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| 作者单位： | 1. 广州中医药大学,广东,广州,510405
2. 清华大学,药物研究所,北京,100084 |
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| 基金项目： | 国家自然科学基金;中国博士后科学基金 |
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| 摘 要： | 目的:建立青藤碱脂质体包封率的测定方法。方法:分别采用凝胶过滤法、微柱离心法、透析法分离脂质体和游离药物,并进行方法学考察,优化包封率测定条件。结果:凝胶过滤法能有效地将青藤碱脂质体与游离青藤碱分离,柱回收率测定结果为98.8%,3次包封率测定的平均值为64.9%,RSD为2.67%。结论:凝胶过滤法较为简便,结果重复性好,适于青藤碱脂质体包封率的测定。
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| 关 键 词： | 青藤碱脂质体 包封率 凝胶过滤法 微柱离心法 透析法 |
| 文章编号： | 1001-5302（2006）09-0731-04 |
| 收稿时间： | 2005-03-11 |
| 修稿时间： | 2005-03-11 |
Study on determination of entrapment efficiency of sinomenine liposomes |
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| ZHOU Li-ling,;WANG Yan,;LIU Qing-fei,;LING Jia-jun.Study on determination of entrapment efficiency of sinomenine liposomes[J].China Journal of Chinese Materia Medica,2006,31(9):731-734. |
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| Authors: | ZHOU Li-ling ;WANG Yan ;LIU Qing-fei ;LING Jia-jun |
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| Institution: | 1. Guangzhou University of Traditional Chinese Medicine, Guru 510405, China; 2. Institute of Materia Medica of Tsinghua University, Beijing 100084, China |
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| Abstract: | Objective: To establish an HPLC method for the determination of entrapment efficiency of sinomenine liposomes. Method: The liposomes and dissociated drugs were separated by sephadex filtration,mini-column centrifugation and dialysis.The methodology study and the optimization of determining condition were carried out at the same time.Result: Sephadex filtration could effectively separate the sinomenine liposomes from dissociated sinomenine.The column recovery was 98.8%,the average entrapment efficiency of three tests was 64.9%,RSD 2.67%.Conclusion: The method was simple,exact,and had a good reappearance.It can be used to examine the entrapment efficiency of sinomenine liposomes. |
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| Keywords: | sinomenine liposomes entrapment efficiency sephadex filtration mini-column centrifugation dialysis |
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