肌苷对大鼠脑缺血再灌注后VEGF表达的影响

①目的　研究肌苷对大鼠脑缺血再灌注后血管内皮生长因子 (VEGF)表达的影响 ,探讨其神经保护作用机制。②方法　成年健康雌性SD大鼠 6 8只 ,应用线栓法建立大鼠脑缺血再灌注动物模型 ,随机分为假手术组 4只 ,肌苷组 32只 ,对照组 32只。肌苷组腹腔注射肌苷 (10 0mg/kg) ,对照组同步注射等量的生理盐水。采用免疫组织化学法检测肌苷对大鼠脑缺血再灌注不同时间内VEGF蛋白表达的影响。③结果　对照组在皮质区和纹状体区于脑缺血再灌注 2hVEGF开始表达 ,12h达高峰 ,持续 2 4h ,随即迅速降低。肌苷治疗组VEGF表达于缺血再灌注 2h～ 2d较对照组显著增高 (t=12 .4 5～ 2 7.78,P <0 .0 1)。④结论　肌苷对脑缺血再灌注后的保护作用可能通过上调脑组织VEGF的表达而实现的。

EFFECTS OF INOSINE ON VEGF EXPRESSION FOLLOWING CEREBRAL ISCHEMIA REPERFUSION IN RATS

ObjectiveTo observe the expression of vascular endothelial growth factor(VEGF) in the cerebral tissue after focal cerebral ischemia reperfusion(CIR) in rats and to explore the neuroprotective effect of inosine on hypoxic-ischemic brain damage. MethodsCIR models were established in 68 healthy adult SD rats, which were divided into the control group and the inosine group. Inosine and same amount of normal saline were injected intraperitoneally into the rats of the inosine group and the controls respectively. Each group was divided into eight subgroups. An immunohistochemical technique was applied to determine VEGF in cerebral tissues. ResultsThe expression of VEGF occurred at two hours after reperfusion, peaked at 12 hours, and then decreased rapidly 24 hours later. At reperfusion of two hours to two days, the level of VEGF in the inosine group was significantly higher than that in the control group (t=12.45-27.78,P<0.01). Conclusion Inosine could prevent hypoxic-ischemic brain damage after reperfusion of focal cerebral ischemia by increasing the expression of VEGF. [