| 利用T─载体克隆法对人脑源性神经营养因子全长基因PCR产物的克隆 |
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| 引用本文： | 余云开,陈谦,赵彬,李兵仓,范明,汪家政. 利用T─载体克隆法对人脑源性神经营养因子全长基因PCR产物的克隆[J]. 军事医学科学院院刊, 1996, 0(1) |
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| 作者姓名： | 余云开 陈谦 赵彬 李兵仓 范明 汪家政 |
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| 作者单位： | 第三军医大学野战外科研所，军事医学科学院基础医学研究所 |
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| 摘 要： | 利用Ｔ－载体克隆法完成了含信号肽及前导肽的人脑源性神经营养因子（ｂｒａｉｎ－ｄｅｒｉｖｅｄｎｅｕｒｏｔｒｏｐｈｉｃｆａｃｔｏｒ，ＢＤＮＦ）全长基因ＰＣＲ产物的克隆。序列测定结果表明，所克隆的人ＢＤＮＦ基因从起始密码子ＡＴＧ到终止密码子ＴＡＧ的７７４ｂｐ中，除下游端ＰＣＲ引物因为采用猪的ＰＣＲ引物而有一个碱基改变外，其它序列与国外文献报道序列完全一致。该碱基改变不影响氨基酸序列
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| 关 键 词： | 神经生长因子；聚合酶链反应；T－载体克隆法；核苷酸 |
Cloning of the PCR products of a full length gene encoding human brain derived neurotrophic factor using the T vector cloning method |
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| Yu Yunkai,Chen Qian,Zhao Bin,Li Bingcang,Fan Ming,Wang Jiazheng Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing. Cloning of the PCR products of a full length gene encoding human brain derived neurotrophic factor using the T vector cloning method[J]. Bulletin of the Academy of Military Medical Sciences, 1996, 0(1) |
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| Authors: | Yu Yunkai Chen Qian Zhao Bin Li Bingcang Fan Ming Wang Jiazheng Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing |
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| Affiliation: | Yu Yunkai,Chen Qian,Zhao Bin,Li Bingcang,Fan Ming,Wang Jiazheng Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850 |
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| Abstract: | By using the T vector cloing method,the authors accomplished the gene cloning of the PCR products of a full length human brain derived neurotrophic factor(BDNF) containing signal peptide and precursor.The cloned human BDNF gene,744 bp-Length from the initiation Coden ATG to the termination Coden TAG,showed similarity to that reported in the foreign literature,except one nucleotide difference at the down stream primer caused by the usage of pig BDNF PCR primer.This alteration did not change the amino sequence. |
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| Keywords: | nerve growth factor polymerase chain reaction T vector cloning method nucleotide |
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