| 小陷胸汤加味含药血清对人脐静脉内皮细胞分泌NO/ET-1的调节作用 |
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| 引用本文： | 喻秀兰,梅国强,张德玲,刘永明,欧阳静萍.小陷胸汤加味含药血清对人脐静脉内皮细胞分泌NO/ET-1的调节作用[J].微循环学杂志,2005,15(2):41-42. |
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| 作者姓名： | 喻秀兰 梅国强 张德玲 刘永明 欧阳静萍 |
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| 作者单位： | 1. 湖北中医学院伤寒教研室,武汉,430061
2. 武汉大学医学院病理生理教研室 |
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| 基金项目： | 武汉市科技计划项目资助 (2 0 0 2 60 0 7171) |
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| 摘 要： | 目的:探讨小陷胸汤加味中药方对血管内皮细胞的保护作用。方港:建立ox-LDL损伤人脐静脉内皮细胞株(ECV-304)模型,用小陷胸汤加味含药血清处理模型,并用放免和硝酸酶还原法在药物干预6h和24h后检测细胞上清液中ET-1和NO含量。结果:100 μg/ml的ox-LDL可损伤血管内皮细胞并导致其分泌NO和ET-1功能失调,小陷胸汤加味含药血清通过影响NO/ET-1的分泌而明显改善此失调状态。结论:小陷胸汤加味中药通过调节NO/ET-1水平显著拮抗ox-LDL对血管内皮细胞损害,具有防治AS的作用。
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| 关 键 词： | 加味小陷胸汤 血管内皮细胞 氧化低密度脂蛋白 人脐静脉 内皮细胞 |
| 文章编号： | 1005-1740(2005)02-0041-02 |
| 修稿时间： | 2004年9月3日 |
Function of Adjustment on the Secretion of NO/ET-1 in HUVEC with the Serum of MD-RPHCA |
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| Yu Xiulan,Mei Guoqiang,Zhang Deling,et al/.Function of Adjustment on the Secretion of NO/ET-1 in HUVEC with the Serum of MD-RPHCA[J].Chinese Journal of Microcirculation,2005,15(2):41-42. |
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| Authors: | Yu Xiulan Mei Guoqiang Zhang Deling / |
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| Institution: | Yu Xiulan,Mei Guoqiang,Zhang Deling,et al/Department of Typhoid Fever,Hubei College of Traditional Chinese Medicine,Wuhan 430061 |
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| Abstract: | Objective: To investigate the protective effect of the minor decoction for removing phlegm-heat from chest with additions(MD-RPHCA) on the vacu-lar endothelial cell(VEC). Method: Established a model with the ECV-304 of human umbilical vein endothelial cell(HUVEC) was injured by ox-LDL and treated by MD-RPHCA, then with the method of NO2 colorimetry and Radio-Immunochemical to measure the level of NO and ET-1 in ECV-304 on the times after 6 h, 24 h. Results: ox-LDL has damaged the VEC and to make the function of secretion on NO,ET-1 derangement but the serum of MD-RPHCA could improve it. Conclusion: Through the regulation of NO/ ET-1, MD-RPHCA maybe antagonism the damage of ox-LDL to the VEC, so that it can prevent and treat the arteriosclerosis. |
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| Keywords: | Minor decoction for removing phlegm-heat from chest with additions(MD-RPHCA) Vaocular endothelial cell (VEC) ox-Low density lipid(ox-LDL) Human umbilical vein endothelial cell(HUVEC) |
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