干细胞移植的磁性标记及磁共振成像活体示踪

干细胞移植的临床应用需要解决植入活体内干细胞在体内存活、迁移及分化的监测问题。通过对干细胞进行顺磁性标记,磁共振成像(MRI)能够在活体上显示标记的干细胞,并进行特异性地追踪及定位,是目前干细胞活体示踪极具前景的方法。干细胞进行磁性标记主要利用铁类或钆类对比剂,两者各有优缺点。利用铁类或钆类对比剂标记干细胞并进行MRI活体监测取得了成功。并在心脑缺血损伤的疾病模型中得到应用,但在干细胞磁性标记的载体选用及其标记率、标记的持久性、标记对细胞活力及遗传性状方面尚存在一定的问题。

Magnetic labelling of transplanted stem cells and in vivo tracking with magnetic resonance imaging

How to monitor and track the survival, migration and differentiation of grafted stem cell in vivo is necessary for the widespread clinically application of stem cell transplantation. Manegtic resonance imaging(MRI), which can display and specifically track and localize labelled stem cell by paramagnetically labelling of the stem cell, is an extremely prospective method to monitor stem cell in vivo. The ferrum and gadlinium derviative contrast agents were mainly used to paramagnetically label the stem cell, and each had its own advantages and disadvatages. Currently, on the basis of paramagnetically labelling of the stem cell with ferrum and gadlinium derviative contrast agents, MRI had been succeessfully used to monitor the stem cell in vivo and had been applied into animal model of the heart and brain stroke, but these are still some problems exist such as the selection of vehicle transferring the contrast agents into stem cells, the labeling efficiency, the duration of labelling and the viablity and heredity changes of the stem cell after labelling.