盐酸戊乙奎醚对哮喘鼠气道平滑肌细胞增殖作用的研究

目的探讨盐酸戊乙奎醚对哮喘鼠气道平滑肌细胞（ASMC）增殖的影响。方法BALB／c小鼠随机分为对照组（A）、哮喘组（B）和盐酸戊乙奎醚干预组（C）。进行支气管肺泡灌洗液（BALF）中细胞分类计数；测定转移生长因子-β1（TGF-β1）的浓度及增殖细胞核抗原（PCNA）的表达；观察肺组织病理形态学变化；图像分析测定支气管基底膜周径（Pbm）、气道壁总面积（WAt）、平滑肌面积（WArn）。结果C组的TGF-β1、PCNA表达及WAt／Pbm、WAm／Pbm比值明显低于B组（P〈0．05），而高于A组（P〈0．05）。结论早期行盐酸戊乙奎醚干预可通过减少TGF-β1在肺组织的表达减轻ASMC增殖。

Research on the effect of penehyclidine hydrochloride inhibiting the proliferation of airway smooth muscle cells of chronic asthma mice

Objective To explore the effects of Penehyclidine Hydrochloride on the proliferation of airway smooth muscle cells（ASMC） of chronic asthma mice. Methods BABL/e mice were divided into 3 groups randomly： group A （control group）, group B （ asthma group）, group C （ Penehyclidine Hydrochloride - intervention group）. Total and differential cell counts in bronchoalveolar lavage fluid （BALF） were observed. To detect the concentration of active TGF -β1 and proliferating cell nuclear antigen （PCNA） and the pathomorphological changes in the lung were analyzed. The perimeter of basement membrane（Pbm） ,the total bronchial wall area （WAt） , the muscle wall area （WAm） were measured by using image analysis system. Results TGF-β1, PCNA level and WAm/Pbm, WArn/Pbm and WAi/Pbm of group C decreased greatly in comparison with group B （ P 〈 0. 05 ）, but increased obviously in comparison with group A （ P 〈 0.05 ）. Conclusion Early intervention with Penehyclidine Hydroehloride might partially inhibits its process via higher expression of TGF -β1 in chronic asthma and decrease the proliferation of ASMC.