Functional Properties of Mesenchymal Stem Cells Labeled with Magnetic Microparticles <Emphasis Type="Italic">in Vitro</Emphasis> and Analysis of Their Distribution after Transplantation |
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| Authors: | A A Konieva I V Kholodenko O A Shragina R V Kholodenko V V Burunova L V Bibaeva K N Yarygin V N Yarygin |
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| Institution: | (1) Department of Surgical Oncology and Technology, Faculty of Medicine, Imperial College London, London, UK;(2) Department of Haematology, Faculty of Medicine, Imperial College London, London, UK;(3) Department of Renal Medicine, Faculty of Medicine, Imperial College London, London, UK |
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| Abstract: | Mesenchymal stem cells enzymatically isolated from human placenta were labeled with magnetic fluorescent microparticles (d=0.96
μ). We showed that microparticles in high doses (>10 μl stock suspension per 1 ml culture medium) significantly inhibited
cell proliferation in culture. In our work we determined the optimal concentration of particles not affecting physiological
properties of mesenchymal stem cells: it does not change cell proliferation, does not induce apoptosis, and does not modulate
their transdifferentiation into neuronal cells. In vivo experiments showed that the chosen particles allow easy visualization of transplanted cells ex vivo on sections of different tissues. |
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