The effect of microRNA-21 on proliferation and matrix synthesis of chondrocytes embedded in atelocollagen gel |
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Authors: | Wirat Kongcharoensombat Tomoyuki Nakasa Masakazu Ishikawa Atsuo Nakamae Masataka Deie Nobuo Adachi Abouheif Mohamed Mitsuo Ochi |
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Institution: | (1) Department of Orthopaedic Surgery, Programs for Applied Biomedicine, Division of Clinical Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan |
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Abstract: | The objective of this study was to investigate the effect of microRNA-21 (miR-21) on the proliferation and matrix synthesis
of chondrocytes embedded in atelocollagen gel. Articular cartilage was harvested aseptically from the knee and hip joints
of rats. In the experimental group, double-stranded miR-21 was transfected into the chondrocytes, and in the control group,
scrambled siRNA was used. After that, chondrocytes were cultured in atelocollagen gel for 3 weeks. At 1, 2, and 3 weeks after
transfection, the cell numbers were counted, and the expression levels of Col2a1 and aggrecan were measured by real-time PCR.
Histological analysis by toluidine blue staining was performed at 3 weeks. The cell number in the experimental group rapidly
increased compared to the control group. The expression levels of Col2a1 and aggrecan in the experimental group were higher
than in the control. Histological analysis revealed that many more cells with a metachromatic stain were present in the experimental
group than in the control. This study demonstrated that miR-21 promotes high proliferation and matrix synthesis of chondrocytes
embedded in atelocollagen gel. |
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