HPLC同时测定妇康片中芍药苷和阿魏酸的含量

目的:建立同时测定妇康片中芍药苷和阿魏酸含量的测定方法.方法:采用Phenomsil C18色谱柱,以乙腈-0.1％磷酸(17∶83)为流动相,流速1.0 mL· min-1,检测波长230 nm.结果:芍药苷在9.3～74.4 mg·L-1呈良好的线性关系(r =0.999 6),平均回收率98.47％,RSD 1.34％.阿魏酸在5.2 ～41.6 mg·L-1呈良好的线性关系(r=0.999 5),平均回收率97.96％,RSD 1.44％.结论:本法简便、灵敏、准确、重复性好,可用于妇康片的质量控制.

Determination of Paeoniflorin and Ferulic Acid in Fukang Tablets by HPLC

Objective:To establish a determination method for paeoniflorin and Ferulic acid in Fukang tablets.Method:The determination was performed on a Phenomsil C18 column with acetonitrile-0.1% phosphoric acid(17∶83)as the mobile phase.The detection wavelength was at 230 nm.Result:Paeoniflorin presented a good linearity in the concentration range of 9.3-74.4 mg·L-1.The average rate of recovery was 98.47% with RSD 1.34%.Ferulic acid presented a good linearity in the concentration range of 5.2-41.6 mg·L-1.The average rate of recovery was 97.96% with RSD 1.44%.Conclusion: This method was simple,accurate and reproducible,it can be used as the basis for quality control of Fukang tablets.